Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WA132_RS11705 Genome accession   NZ_CP146939
Coordinates   2479214..2479891 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain 2A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2474214..2484891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA132_RS11690 (WA132_11690) chiA 2475573..2478275 (+) 2703 WP_001765222.1 bifunctional chitinase/lysozyme -
  WA132_RS11695 (WA132_11695) bfd 2478443..2478637 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WA132_RS11700 (WA132_11700) bfr 2478709..2479185 (+) 477 WP_000675504.1 bacterioferritin -
  WA132_RS11705 (WA132_11705) pilD 2479214..2479891 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  WA132_RS11710 (WA132_11710) gspM 2479891..2480352 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  WA132_RS11715 (WA132_11715) gspL 2480349..2481512 (-) 1164 WP_148983764.1 type II secretion system protein GspL -
  WA132_RS11720 (WA132_11720) gspK 2481527..2482510 (-) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  WA132_RS11725 (WA132_11725) gspJ 2482503..2483090 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WA132_RS11730 (WA132_11730) gspI 2483083..2483460 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  WA132_RS11735 (WA132_11735) gspH 2483457..2483966 (-) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  WA132_RS11740 (WA132_11740) gspG 2483974..2484411 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=857372 WA132_RS11705 WP_000178175.1 2479214..2479891(-) (pilD) [Escherichia coli strain 2A]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=857372 WA132_RS11705 WP_000178175.1 2479214..2479891(-) (pilD) [Escherichia coli strain 2A]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387