Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V9Z41_RS01975 Genome accession   NZ_CP146679
Coordinates   426583..427260 (-) Length   225 a.a.
NCBI ID   WP_000178177.1    Uniprot ID   -
Organism   Escherichia coli strain MS 200-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421583..432260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9Z41_RS01960 (V9Z41_01960) chiA 422941..425643 (+) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -
  V9Z41_RS01965 (V9Z41_01965) bfd 425812..426006 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V9Z41_RS01970 (V9Z41_01970) bfr 426078..426554 (+) 477 WP_000675504.1 bacterioferritin -
  V9Z41_RS01975 (V9Z41_01975) pilD 426583..427260 (-) 678 WP_000178177.1 prepilin peptidase GspO Machinery gene
  V9Z41_RS01980 (V9Z41_01980) gspM 427260..427721 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  V9Z41_RS01985 (V9Z41_01985) gspL 427718..428881 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  V9Z41_RS01990 (V9Z41_01990) gspK 428896..429879 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  V9Z41_RS01995 (V9Z41_01995) gspJ 429872..430459 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  V9Z41_RS02000 (V9Z41_02000) gspI 430452..430847 (-) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  V9Z41_RS02005 (V9Z41_02005) gspH 430844..431353 (-) 510 WP_001076037.1 type II secretion system minor pseudopilin GspH -
  V9Z41_RS02010 (V9Z41_02010) gspG 431361..431798 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24900.77 Da        Isoelectric Point: 7.6364

>NTDB_id=856854 V9Z41_RS01975 WP_000178177.1 426583..427260(-) (pilD) [Escherichia coli strain MS 200-1]
MTMLLPLFILVGFIASYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=856854 V9Z41_RS01975 WP_000178177.1 426583..427260(-) (pilD) [Escherichia coli strain MS 200-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAAGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382