Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V9Z40_RS01485 Genome accession   NZ_CP146667
Coordinates   316389..317885 (+) Length   498 a.a.
NCBI ID   WP_001040643.1    Uniprot ID   -
Organism   Escherichia coli strain MS 16-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 311389..322885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9Z40_RS01455 (V9Z40_01455) tusA 311469..311714 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  V9Z40_RS01460 (V9Z40_01460) zntA 311983..314181 (-) 2199 WP_000106592.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  V9Z40_RS01465 (V9Z40_01465) yhhN 314255..314881 (-) 627 WP_000964718.1 lysoplasmalogenase -
  V9Z40_RS01470 (V9Z40_01470) yhhM 315022..315381 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  V9Z40_RS01475 (V9Z40_01475) yhhL 315384..315653 (-) 270 WP_001295207.1 DUF1145 family protein -
  V9Z40_RS01480 (V9Z40_01480) rsmD 315643..316239 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  V9Z40_RS01485 (V9Z40_01485) pilA 316389..317885 (+) 1497 WP_001040643.1 signal recognition particle-docking protein FtsY Machinery gene
  V9Z40_RS01490 (V9Z40_01490) ftsE 317888..318556 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  V9Z40_RS01495 (V9Z40_01495) ftsX 318549..319607 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  V9Z40_RS01500 (V9Z40_01500) rpoH 319852..320706 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  V9Z40_RS01505 (V9Z40_01505) livJ 320977..322080 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  V9Z40_RS01510 (V9Z40_01510) panM 322268..322651 (-) 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54623.42 Da        Isoelectric Point: 4.1810

>NTDB_id=856597 V9Z40_RS01485 WP_001040643.1 316389..317885(+) (pilA) [Escherichia coli strain MS 16-3]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAANVVDVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=856597 V9Z40_RS01485 WP_001040643.1 316389..317885(+) (pilA) [Escherichia coli strain MS 16-3]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCAACGTTGTGGACGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGTCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCTGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378