Detailed information NTG0085630

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: DHCNIT_RS00280
• Genome accession: NZ_AP024514
• Coordinates: 54510..55904 (+)
• Length: 464 a.a.
• NCBI ID: WP_034376942.1
• Uniprot ID: A0A142V8C5
• Organism: Dehalococcoides mccartyi strain NIT01
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49510..60904

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DHCNIT_RS00275 (DHCNIT_000540)	-	51589..54063 (+)	2475	WP_011308690.1	ATP-dependent Clp protease ATP-binding subunit	-
DHCNIT_RS00280 (DHCNIT_000550)	radA	54510..55904 (+)	1395	WP_034376942.1	DNA repair protein RadA	Machinery gene
DHCNIT_RS00285 (DHCNIT_000560)	-	55891..56574 (+)	684	WP_011308692.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
DHCNIT_RS00290 (DHCNIT_000570)	ispF	56580..57053 (+)	474	WP_011308693.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
DHCNIT_RS00295 (DHCNIT_000580)	cysS	57057..58430 (+)	1374	WP_011308694.1	cysteine--tRNA ligase	-

Sequence

Protein

• Length: 464 a.a.
• Molecular weight: 49403.74 Da
• Isoelectric Point: 6.5004

>NTDB_id=85630 DHCNIT_RS00280 WP_034376942.1 54510..55904(+) (radA) [Dehalococcoides mccartyi strain NIT01]
MDKSRNVYICSNCGHESLKWLGRCPGCQEWNTLEETTIAAPLGRKNAPARVISPAAELSSLNASDTTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKNSGASVFIAAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLIRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTIMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFTRALVPAGANCQNININGIQIIAVSTVKEAIKLALTGVKTETEDVFE

Nucleotide

• Length: 1395 bp

>NTDB_id=85630 DHCNIT_RS00280 WP_034376942.1 54510..55904(+) (radA) [Dehalococcoides mccartyi strain NIT01]
ATGGATAAAAGCCGTAATGTTTATATCTGTTCCAACTGCGGACATGAGAGCCTGAAGTGGCTGGGGCGTTGCCCCGGCTG
TCAGGAATGGAATACTCTTGAAGAAACAACAATTGCTGCACCTCTCGGGCGTAAAAATGCACCTGCCCGGGTTATCAGCC
CGGCAGCCGAGCTTTCCAGTCTGAATGCATCAGATACTACCCGCCGGAGTCTATCTATTTCAGAGTTCAACCGGGTTTTG
GGCGGTGGAATAGTGCCGGGTTCACTGATGCTTTTAGGGGGTGAACCGGGTATCGGCAAATCCACGCTCCTTCTTCAGGT
GGCTGCCTCGGTAGCCCAGAGCGGCGGCAAAGTGGTATATGTTTCCGGGGAAGAGAACCCCGCCCAGATAAAAATGCGCG
CTCAGCGTCTGGGTATCAGCGGCGAGGGGCTTTTCCTTATGGCTGAGACAGACCTTAATGCCATTCTAGCCCAGCTTTCA
GTCCTCTGCCCGTCATTGGTTGTTATAGACTCTATCCAGACTGTATTCCTGCCTGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTGATGCAGTGGGCTAAGAACAGCGGTGCCAGCGTATTTATTGCCGCCCATG
TTACCAAAGAGGGCAACATCGCCGGGCCGCGCATACTGGAACATATAGTAGATGTGGTCATGTACTTTGAGGGCGAGTCC
CAGAGTGCTTACCGTTTGATACGTTCGGTCAAAAACCGCTTCGGTTCTACCAACGAAGTAGGCATATTTGAAATGAAAAG
TGAAGGATTGGTGGAAGTAGCCAATCCCTCGCAGATATTTTTATCTAATCGGCAGGCAAACACTGTTGGTTCAGCTGTAA
CAGCAGTGCTGGAGGGTAGCCGCCCTTTACTGGTAGAGGTGCAGGCACTCACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACGGCCAACGGGGTAGATTTTAACCGTACTATTATGATAGCCGCTGTTCTTTCCAAACGCCTTTCCATGCGGCTGGG
TACCCAGGACATAATAGTAAATGCCACCGGCGGTATTCGTCTGGACGAGCCGGCCGCAGATTTGGCTATTGCTTTGGCCA
TTGCCTCCAGTTACCGTGATATCGGGGTCTGCCCGGAAACCATAGCACTGGGTGAGATTGGCCTTTCAGGGGAACTGAGG
ACAATCCCTCATTTGGAAAGACGTCTTTCCGAGGCCAGCCGTTTGGGTTTTACGAGGGCTTTGGTACCTGCCGGTGCTAA
TTGCCAGAATATAAATATAAATGGTATCCAGATTATTGCGGTTTCAACTGTCAAAGAGGCTATTAAACTGGCGCTTACCG
GGGTAAAAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (335-424)

• AAA domain (AAA_25) (66-218)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

3D structure

• AlphaFold DB: A0A142V8C5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	50.218	98.707	0.496
radA	Streptococcus pneumoniae Rx1	48.578	98.491	0.478
radA	Streptococcus pneumoniae D39	48.578	98.491	0.478
radA	Streptococcus pneumoniae R6	48.578	98.491	0.478
radA	Streptococcus pneumoniae TIGR4	48.578	98.491	0.478
radA	Streptococcus mitis SK321	48.578	98.491	0.478
radA	Streptococcus mitis NCTC 12261	48.359	98.491	0.476