Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V9K85_RS02045 Genome accession   NZ_CP146650
Coordinates   438670..439335 (-) Length   221 a.a.
NCBI ID   WP_000178160.1    Uniprot ID   -
Organism   Escherichia coli strain MS 85-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433670..444335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9K85_RS02025 (V9K85_02025) tuf 433858..435042 (+) 1185 WP_000031783.1 elongation factor Tu -
  V9K85_RS02030 (V9K85_02030) - 435334..437718 (+) 2385 WP_000773136.1 carbohydrate-binding protein -
  V9K85_RS02035 (V9K85_02035) bfd 437887..438081 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V9K85_RS02040 (V9K85_02040) bfr 438153..438629 (+) 477 WP_000675504.1 bacterioferritin -
  V9K85_RS02045 (V9K85_02045) pilD 438670..439335 (-) 666 WP_000178160.1 prepilin peptidase GspO Machinery gene
  V9K85_RS02050 (V9K85_02050) gspM 439335..439796 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  V9K85_RS02055 (V9K85_02055) gspL 439793..440956 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  V9K85_RS02060 (V9K85_02060) gspK 440971..441954 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V9K85_RS02065 (V9K85_02065) gspJ 441947..442534 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V9K85_RS02070 (V9K85_02070) gspI 442527..442904 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V9K85_RS02075 (V9K85_02075) gspH 442901..443410 (-) 510 WP_001076047.1 type II secretion system minor pseudopilin GspH -
  V9K85_RS02080 (V9K85_02080) gspG 443418..443855 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 24349.16 Da        Isoelectric Point: 7.9585

>NTDB_id=856022 V9K85_RS02045 WP_000178160.1 438670..439335(-) (pilD) [Escherichia coli strain MS 85-1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLA

Nucleotide


Download         Length: 666 bp        

>NTDB_id=856022 V9K85_RS02045 WP_000178160.1 438670..439335(-) (pilD) [Escherichia coli strain MS 85-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTAG

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.389