Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V8947_RS26540 Genome accession   NZ_CP146595
Coordinates   5129603..5130043 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain A1.S1.18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5124603..5135043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8947_RS26515 (V8947_26515) coaE 5125093..5125713 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  V8947_RS26520 (V8947_26520) - 5125738..5125782 (+) 45 WP_120795372.1 protein YacM -
  V8947_RS26525 (V8947_26525) guaC 5125938..5126981 (+) 1044 WP_001217320.1 GMP reductase -
  V8947_RS26530 (V8947_26530) hofC 5127016..5128218 (-) 1203 WP_000157289.1 protein transport protein HofC -
  V8947_RS26535 (V8947_26535) gspE 5128208..5129593 (-) 1386 Protein_4999 type II secretion system protein GspE -
  V8947_RS26540 (V8947_26540) pilA 5129603..5130043 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  V8947_RS26545 (V8947_26545) nadC 5130247..5131140 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V8947_RS26550 (V8947_26550) ampD 5131228..5131779 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V8947_RS26555 (V8947_26555) ampE 5131776..5132629 (+) 854 Protein_5003 beta-lactamase regulator AmpE -
  V8947_RS26560 (V8947_26560) aroP 5132672..5134042 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=856012 V8947_RS26540 WP_000360914.1 5129603..5130043(-) (pilA) [Escherichia coli strain A1.S1.18]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=856012 V8947_RS26540 WP_000360914.1 5129603..5130043(-) (pilA) [Escherichia coli strain A1.S1.18]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363