Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QYQ99_RS13785 Genome accession   NZ_CP129672
Coordinates   3000760..3002361 (+) Length   533 a.a.
NCBI ID   WP_302088696.1    Uniprot ID   -
Organism   Comamonas testosteroni strain MWF001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2995760..3007361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ99_RS13760 (QYQ99_13760) argS 2995780..2997489 (+) 1710 WP_302088692.1 arginine--tRNA ligase -
  QYQ99_RS13765 (QYQ99_13765) - 2997590..2998285 (+) 696 WP_302088693.1 SPOR domain-containing protein -
  QYQ99_RS13770 (QYQ99_13770) - 2998398..2999048 (+) 651 WP_302088694.1 thiol:disulfide interchange protein DsbA/DsbL -
  QYQ99_RS13775 (QYQ99_13775) lptA 2999179..2999835 (+) 657 WP_302088695.1 lipopolysaccharide transport periplasmic protein LptA -
  QYQ99_RS13780 (QYQ99_13780) lptB 2999832..3000614 (+) 783 WP_003060565.1 LPS export ABC transporter ATP-binding protein -
  QYQ99_RS13785 (QYQ99_13785) rpoN/rpoN1 3000760..3002361 (+) 1602 WP_302088696.1 RNA polymerase factor sigma-54 Machinery gene
  QYQ99_RS13790 (QYQ99_13790) - 3002537..3003457 (-) 921 WP_302088697.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  QYQ99_RS13795 (QYQ99_13795) - 3003591..3004823 (-) 1233 WP_302088698.1 DUF711 family protein -
  QYQ99_RS13800 (QYQ99_13800) - 3004881..3005219 (-) 339 WP_053283100.1 tRNA-binding protein -
  QYQ99_RS13805 (QYQ99_13805) - 3005399..3006697 (+) 1299 WP_302088699.1 class I SAM-dependent RNA methyltransferase -
  QYQ99_RS13810 (QYQ99_13810) - 3006694..3007188 (+) 495 WP_302088700.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59020.38 Da        Isoelectric Point: 4.6077

>NTDB_id=854854 QYQ99_RS13785 WP_302088696.1 3000760..3002361(+) (rpoN/rpoN1) [Comamonas testosteroni strain MWF001]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPVDVAASAGEYSTSDSIHAE
KESLQAQPATETVAEASTPETPETLNWEADGMDGQDGEWGGESSSEWDAPSASSSTRNRDGDDETDAIDLACEHQSLTDF
LHRQALSLHLGAADMAALRFLIESLSEDGYLEDSLEELAQSLAGDDLEEREELEQHFVIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRNAEDEGTTEAHMNRLLLAQSICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVIPDVIVRPVRAGSGRADFSVQLNPDVMPRLRVHDIYASVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDAKKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=854854 QYQ99_RS13785 WP_302088696.1 3000760..3002361(+) (rpoN/rpoN1) [Comamonas testosteroni strain MWF001]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTGTCGCTTACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAGCGTGCGGGCGATGAGG
CCGAAACCGCAGGAGATGCTCCTGTAGATGTAGCTGCTAGCGCCGGTGAATACAGCACTTCAGATAGTATTCATGCTGAA
AAGGAGTCATTGCAAGCGCAACCAGCTACTGAAACGGTAGCGGAGGCGTCAACCCCCGAAACCCCTGAAACGCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGGGAGTGGGGCGGTGAATCGAGCAGCGAGTGGGATGCGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGTGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCGGACATGGCGGCACTGCGCTTTCTCATCGAGTCGCTCAGCGA
GGACGGCTATCTCGAGGATTCCCTGGAGGAGCTGGCGCAGAGCCTGGCCGGCGACGACCTGGAAGAGCGCGAGGAGCTGG
AGCAGCACTTTGTCATTGCTCTGAAGCTGCTGCAAAGCCTGGAGCCCGTGGGCGTGGGGGCGCGTGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGACCTGATCCGCAACGCCGAGGACGAGGGCACGACCGAGGCGCATATGAACCGGTTGCTGCT
GGCGCAATCCATCTGCGCGCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGTGGCGCAC
GCGAGGAGCAACTGCGCGAAGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGGCGCCGCTTTGTCGACGTGGAA
CGCCATATCGTGATACCCGATGTCATCGTGCGTCCGGTGCGGGCAGGCTCGGGCCGGGCGGACTTTTCCGTGCAGCTCAA
CCCCGATGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCAGCGTGCTGCGTCGCCACAAGAACAGCGACGGTCATG
CGGCGCTGCAGCAGCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGGCCGCTGGTGCTGCGCGATAT
CGCCGACACGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAAGGCACCT
TCGAGCTCAAGTATTTCTTCGGCTCGGGCCTGGGTACGGAAACCGGCGGCAGTGCCTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTTGTCGATGCCGAAGATGCCAAAAAGCCGCTATCGGATGCAAAACTAGCCGACATGCTCAAGGAGCAGGG
TATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.124

100

0.512