Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V6591_RS02560 Genome accession   NZ_CP146019
Coordinates   533308..534741 (-) Length   477 a.a.
NCBI ID   WP_000809047.1    Uniprot ID   -
Organism   Escherichia coli strain ECO8855     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528308..539741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6591_RS02525 (V6591_02530) elbB 528999..529652 (+) 654 WP_001307414.1 isoprenoid biosynthesis glyoxalase ElbB -
  V6591_RS02530 (V6591_02535) mtgA 529649..530377 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V6591_RS02535 (V6591_02540) yrbL 530374..531006 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  V6591_RS02540 (V6591_02545) npr 531220..531492 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  V6591_RS02545 (V6591_02550) rapZ 531489..532343 (-) 855 WP_000243741.1 RNase adapter RapZ -
  V6591_RS02550 (V6591_02555) ptsN 532389..532880 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V6591_RS02555 (V6591_02560) hpf 532998..533285 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  V6591_RS02560 (V6591_02565) rpoN/rpoN1 533308..534741 (-) 1434 WP_000809047.1 RNA polymerase factor sigma-54 Machinery gene
  V6591_RS02565 (V6591_02570) lptB 534789..535514 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  V6591_RS02570 (V6591_02575) lptA 535521..536078 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V6591_RS02575 (V6591_02580) lptC 536047..536622 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  V6591_RS02580 (V6591_02585) kdsC 536619..537185 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V6591_RS02585 (V6591_02590) kdsD 537206..538192 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  V6591_RS02590 (V6591_02595) yrbG 538206..539183 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54051.83 Da        Isoelectric Point: 4.3447

>NTDB_id=854477 V6591_RS02560 WP_000809047.1 533308..534741(-) (rpoN/rpoN1) [Escherichia coli strain ECO8855]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=854477 V6591_RS02560 WP_000809047.1 533308..534741(-) (rpoN/rpoN1) [Escherichia coli strain ECO8855]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCAACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGTTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGATTACATTGACGA
CGAGCTGCCTGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTAACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGATGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465