Detailed information NTG0854469

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: V6591_RS00960
• Genome accession: NZ_CP146019
• Coordinates: 204946..205929 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain ECO8855
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 199946..210929

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6591_RS00945 (V6591_00945)	dppA	201246..202853 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
V6591_RS00950 (V6591_00950)	dppB	203004..204023 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
V6591_RS00955 (V6591_00955)	dppC	204033..204935 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
V6591_RS00960 (V6591_00960)	amiE	204946..205929 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
V6591_RS00965 (V6591_00965)	dppF	205926..206930 (+)	1005	WP_000107030.1	dipeptide ABC transporter ATP-binding subunit DppF	-
V6591_RS00970 (V6591_00970)	yhjV	206960..208231 (-)	1272	WP_001402620.1	aromatic amino acid transport family protein	-
V6591_RS00975 (V6591_00975)	ldrD	208707..208814 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
V6591_RS00980 (V6591_00980)	-	209190..209297 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
V6591_RS00985 (V6591_00985)	-	209673..209780 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=854469 V6591_RS00960 WP_001196486.1 204946..205929(+) (amiE) [Escherichia coli strain ECO8855]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=854469 V6591_RS00960 WP_001196486.1 204946..205929(+) (amiE) [Escherichia coli strain ECO8855]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCTGGCGGTATGAGTCAG
CGTGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGTTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGTAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGATGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404