Detailed information NTG0854424

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: V6773_RS12995
• Genome accession: NZ_CP145946
• Coordinates: 2511469..2512452 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli PA8
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 2506469..2517452

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6773_RS12970 (V6773_12970)	bcsF	2506630..2506821 (+)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
V6773_RS12975 (V6773_12975)	bcsG	2506818..2508497 (+)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
V6773_RS12980 (V6773_12980)	ldrD	2508584..2508691 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
V6773_RS12985 (V6773_12985)	yhjV	2509167..2510438 (+)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
V6773_RS12990 (V6773_12990)	dppF	2510468..2511472 (-)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
V6773_RS12995 (V6773_12995)	amiE	2511469..2512452 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
V6773_RS13000 (V6773_13000)	dppC	2512463..2513365 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
V6773_RS13005 (V6773_13005)	dppB	2513375..2514394 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
V6773_RS13010 (V6773_13010)	dppA	2514545..2516152 (-)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=854424 V6773_RS12995 WP_001196486.1 2511469..2512452(-) (amiE) [Escherichia coli PA8]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=854424 V6773_RS12995 WP_001196486.1 2511469..2512452(-) (amiE) [Escherichia coli PA8]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404