Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUE37_RS05695 Genome accession   NZ_AP027404
Coordinates   1009681..1010358 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EC416 isolate EC416     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1004681..1015358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE37_RS05680 (MUTS2_10820) chiA 1006048..1008741 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  QUE37_RS05685 (MUTS2_10830) bfd 1008910..1009104 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUE37_RS05690 (MUTS2_10840) bfr 1009176..1009652 (+) 477 WP_000675504.1 bacterioferritin -
  QUE37_RS05695 (MUTS2_10850) pilD 1009681..1010358 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QUE37_RS05700 (MUTS2_10860) gspM 1010358..1010819 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  QUE37_RS05705 (MUTS2_10870) gspL 1010816..1011979 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  QUE37_RS05710 (MUTS2_10880) gspK 1011994..1012977 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QUE37_RS05715 (MUTS2_10890) gspJ 1012970..1013530 (-) 561 WP_286347793.1 type II secretion system minor pseudopilin GspJ -
  QUE37_RS05720 (MUTS2_10900) gspI 1013523..1013900 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QUE37_RS05725 (MUTS2_10910) gspH 1013897..1014406 (-) 510 WP_286347794.1 type II secretion system minor pseudopilin GspH -
  QUE37_RS05730 (MUTS2_10920) gspG 1014414..1014851 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=85401 QUE37_RS05695 WP_000178161.1 1009681..1010358(-) (pilD) [Escherichia coli strain EC416 isolate EC416]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=85401 QUE37_RS05695 WP_000178161.1 1009681..1010358(-) (pilD) [Escherichia coli strain EC416 isolate EC416]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment