Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R2TS_RS20030 Genome accession   NZ_AP024498
Coordinates   4157847..4159280 (+) Length   477 a.a.
NCBI ID   WP_047060252.1    Uniprot ID   -
Organism   Enterobacter asburiae strain En30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4152847..4164280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2TS_RS20000 (R2TS_39130) - 4153414..4154391 (+) 978 WP_039260412.1 calcium/sodium antiporter -
  R2TS_RS20005 (R2TS_39140) kdsD 4154405..4155391 (+) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  R2TS_RS20010 (R2TS_39150) kdsC 4155406..4155972 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  R2TS_RS20015 (R2TS_39160) lptC 4155969..4156544 (+) 576 WP_182071509.1 LPS export ABC transporter periplasmic protein LptC -
  R2TS_RS20020 (R2TS_39170) lptA 4156513..4157067 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  R2TS_RS20025 (R2TS_39180) lptB 4157074..4157799 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  R2TS_RS20030 (R2TS_39190) rpoN/rpoN1 4157847..4159280 (+) 1434 WP_047060252.1 RNA polymerase factor sigma-54 Machinery gene
  R2TS_RS20035 (R2TS_39200) hpf 4159303..4159590 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  R2TS_RS20040 (R2TS_39210) ptsN 4159673..4160164 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  R2TS_RS20045 (R2TS_39220) rapZ 4160210..4161064 (+) 855 WP_014885397.1 RNase adapter RapZ -
  R2TS_RS20050 (R2TS_39230) npr 4161061..4161333 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  R2TS_RS20055 (R2TS_39240) mtgA 4161373..4162098 (-) 726 WP_182071508.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  R2TS_RS20060 (R2TS_39250) elbB 4162095..4162748 (-) 654 WP_182071536.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3173

>NTDB_id=85389 R2TS_RS20030 WP_047060252.1 4157847..4159280(+) (rpoN/rpoN1) [Enterobacter asburiae strain En30]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=85389 R2TS_RS20030 WP_047060252.1 4157847..4159280(+) (rpoN/rpoN1) [Enterobacter asburiae strain En30]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATTGCTACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGAAGAGATTGAACTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCCCGCCCGGGACAGTCGATCCAAACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCGAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment