Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QOM25_RS08150 Genome accession   NZ_CP129497
Coordinates   1675342..1676775 (-) Length   477 a.a.
NCBI ID   WP_090418872.1    Uniprot ID   -
Organism   Enterobacter asburiae strain MP4m4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1670342..1681775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOM25_RS08120 (QOM25_08120) elbB 1671874..1672527 (+) 654 WP_301512903.1 isoprenoid biosynthesis glyoxalase ElbB -
  QOM25_RS08125 (QOM25_08125) mtgA 1672524..1673249 (+) 726 WP_045355397.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QOM25_RS08130 (QOM25_08130) npr 1673289..1673561 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  QOM25_RS08135 (QOM25_08135) rapZ 1673558..1674412 (-) 855 WP_014885397.1 RNase adapter RapZ -
  QOM25_RS08140 (QOM25_08140) ptsN 1674458..1674949 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QOM25_RS08145 (QOM25_08145) hpf 1675032..1675319 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  QOM25_RS08150 (QOM25_08150) rpoN/rpoN1 1675342..1676775 (-) 1434 WP_090418872.1 RNA polymerase factor sigma-54 Machinery gene
  QOM25_RS08155 (QOM25_08155) lptB 1676823..1677548 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  QOM25_RS08160 (QOM25_08160) lptA 1677555..1678109 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QOM25_RS08165 (QOM25_08165) lptC 1678078..1678653 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  QOM25_RS08170 (QOM25_08170) kdsC 1678650..1679216 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QOM25_RS08175 (QOM25_08175) kdsD 1679231..1680217 (-) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  QOM25_RS08180 (QOM25_08180) - 1680231..1681208 (-) 978 WP_023309354.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3149

>NTDB_id=853636 QOM25_RS08150 WP_090418872.1 1675342..1676775(-) (rpoN/rpoN1) [Enterobacter asburiae strain MP4m4]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=853636 QOM25_RS08150 WP_090418872.1 1675342..1676775(-) (rpoN/rpoN1) [Enterobacter asburiae strain MP4m4]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACGCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCTAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTGATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48