Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QYM03_RS06390 Genome accession   NZ_CP129401
Coordinates   1421100..1422212 (+) Length   370 a.a.
NCBI ID   WP_025887558.1    Uniprot ID   A0A379YLK5
Organism   Shewanella algae strain MCCC 1A11468     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1416100..1427212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYM03_RS06360 (QYM03_06360) - 1417011..1417445 (-) 435 WP_025887551.1 DUF4426 domain-containing protein -
  QYM03_RS06365 (QYM03_06365) yggU 1417508..1417795 (-) 288 WP_037467122.1 DUF167 family protein YggU -
  QYM03_RS06370 (QYM03_06370) - 1417795..1418346 (-) 552 WP_234520359.1 YggT family protein -
  QYM03_RS06375 (QYM03_06375) proC 1418370..1419188 (-) 819 WP_234520365.1 pyrroline-5-carboxylate reductase -
  QYM03_RS06380 (QYM03_06380) - 1419321..1420016 (-) 696 WP_301561278.1 YggS family pyridoxal phosphate-dependent enzyme -
  QYM03_RS06385 (QYM03_06385) pilT 1420053..1421090 (+) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  QYM03_RS06390 (QYM03_06390) pilU 1421100..1422212 (+) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QYM03_RS06395 (QYM03_06395) - 1422337..1423446 (+) 1110 WP_234522207.1 hypothetical protein -
  QYM03_RS06400 (QYM03_06400) - 1423486..1424067 (+) 582 WP_301561279.1 glutathione peroxidase -
  QYM03_RS06405 (QYM03_06405) hemH 1424102..1425115 (+) 1014 WP_301561280.1 ferrochelatase -
  QYM03_RS06410 (QYM03_06410) - 1425287..1426021 (+) 735 WP_044733990.1 tryptophan 2,3-dioxygenase family protein -
  QYM03_RS06415 (QYM03_06415) - 1426032..1427162 (+) 1131 WP_301561281.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3709

>NTDB_id=853005 QYM03_RS06390 WP_025887558.1 1421100..1422212(+) (pilU) [Shewanella algae strain MCCC 1A11468]
MEVRPFLKAMVDRKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKDTMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=853005 QYM03_RS06390 WP_025887558.1 1421100..1422212(+) (pilU) [Shewanella algae strain MCCC 1A11468]
ATGGAAGTCAGACCTTTTCTCAAGGCTATGGTGGATCGCAAGGCCTCGGATCTCTTTATTACCGCCGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGCTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAAGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGTATCGAAACCAAGATCCCTACTGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGACTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTAGGGGGAACCGGTA
CGGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCCAATGCCAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCACCAACAACTGCTATTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCAGTGGTCGTCGCGCCGCCATCGAAGTGCTGATCAACACCCCCAGGGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGACACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
TTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCCGACTCGCCAAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAACTCAGGTTTCATGGAGGGAGTCACCCTGGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379YLK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

59.375

95.135

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362