Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V6S24_RS03465 Genome accession   NZ_CP145718
Coordinates   428134..428811 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain S1-CAM-01-C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423134..433811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S24_RS03450 (V6S24_03450) chiA 424501..427194 (+) 2694 WP_260232507.1 bifunctional chitinase/lysozyme -
  V6S24_RS03455 (V6S24_03455) bfd 427363..427557 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V6S24_RS03460 (V6S24_03460) bfr 427629..428105 (+) 477 WP_000675504.1 bacterioferritin -
  V6S24_RS03465 (V6S24_03465) pilD 428134..428811 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  V6S24_RS03470 (V6S24_03470) gspM 428811..429272 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  V6S24_RS03475 (V6S24_03475) gspL 429269..430432 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  V6S24_RS03480 (V6S24_03480) gspK 430447..431430 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V6S24_RS03485 (V6S24_03485) gspJ 431423..432010 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V6S24_RS03490 (V6S24_03490) gspI 432003..432380 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V6S24_RS03495 (V6S24_03495) gspH 432377..432886 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  V6S24_RS03500 (V6S24_03500) gspG 432894..433331 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=852818 V6S24_RS03465 WP_000178161.1 428134..428811(-) (pilD) [Escherichia coli strain S1-CAM-01-C]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=852818 V6S24_RS03465 WP_000178161.1 428134..428811(-) (pilD) [Escherichia coli strain S1-CAM-01-C]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACTTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378