Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V6S25_RS04870 Genome accession   NZ_CP145714
Coordinates   475425..476102 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain S1-CAM-01-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470425..481102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S25_RS04855 (V6S25_04855) chiA 471792..474485 (+) 2694 WP_032255612.1 bifunctional chitinase/lysozyme -
  V6S25_RS04860 (V6S25_04860) bfd 474654..474848 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V6S25_RS04865 (V6S25_04865) bfr 474920..475396 (+) 477 WP_000675504.1 bacterioferritin -
  V6S25_RS04870 (V6S25_04870) pilD 475425..476102 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  V6S25_RS04875 (V6S25_04875) gspM 476102..476563 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  V6S25_RS04880 (V6S25_04880) gspL 476560..477723 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  V6S25_RS04885 (V6S25_04885) gspK 477738..478721 (-) 984 WP_273683482.1 type II secretion system minor pseudopilin GspK -
  V6S25_RS04890 (V6S25_04890) gspJ 478714..479301 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V6S25_RS04895 (V6S25_04895) gspI 479294..479671 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V6S25_RS04900 (V6S25_04900) gspH 479668..480177 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  V6S25_RS04905 (V6S25_04905) gspG 480185..480622 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=852739 V6S25_RS04870 WP_000178161.1 475425..476102(-) (pilD) [Escherichia coli strain S1-CAM-01-A]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=852739 V6S25_RS04870 WP_000178161.1 475425..476102(-) (pilD) [Escherichia coli strain S1-CAM-01-A]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378