Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V6S26_RS05265 Genome accession   NZ_CP145707
Coordinates   763010..763450 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain S1-CAM-02-A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 758010..768450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S26_RS05240 (V6S26_05240) coaE 758500..759120 (-) 621 WP_077501482.1 dephospho-CoA kinase -
  V6S26_RS05245 (V6S26_05245) - 759145..759189 (+) 45 WP_120795372.1 protein YacM -
  V6S26_RS05250 (V6S26_05250) guaC 759345..760388 (+) 1044 WP_001217338.1 GMP reductase -
  V6S26_RS05255 (V6S26_05255) hofC 760423..761625 (-) 1203 WP_000157234.1 protein transport protein HofC -
  V6S26_RS05260 (V6S26_05260) pilB 761615..763000 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  V6S26_RS05265 (V6S26_05265) pilA 763010..763450 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  V6S26_RS05270 (V6S26_05270) nadC 763653..764546 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6S26_RS05275 (V6S26_05275) ampD 764634..765185 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6S26_RS05280 (V6S26_05280) ampE 765182..766036 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  V6S26_RS05285 (V6S26_05285) aroP 766079..767452 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=852665 V6S26_RS05265 WP_000360895.1 763010..763450(-) (pilA) [Escherichia coli strain S1-CAM-02-A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=852665 V6S26_RS05265 WP_000360895.1 763010..763450(-) (pilA) [Escherichia coli strain S1-CAM-02-A]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363