Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V6S27_RS19325 Genome accession   NZ_CP145703
Coordinates   3820958..3821398 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain S1-CAM-04-C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3815958..3826398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S27_RS19300 (V6S27_19300) coaE 3816448..3817068 (-) 621 WP_001269513.1 dephospho-CoA kinase -
  V6S27_RS19305 (V6S27_19305) - 3817093..3817137 (+) 45 WP_120795372.1 protein YacM -
  V6S27_RS19310 (V6S27_19310) guaC 3817293..3818336 (+) 1044 WP_001217337.1 GMP reductase -
  V6S27_RS19315 (V6S27_19315) hofC 3818371..3819573 (-) 1203 WP_000157287.1 protein transport protein HofC -
  V6S27_RS19320 (V6S27_19320) pilB 3819563..3820948 (-) 1386 WP_001025168.1 type II secretion system protein GspE Machinery gene
  V6S27_RS19325 (V6S27_19325) pilA 3820958..3821398 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  V6S27_RS19330 (V6S27_19330) nadC 3821601..3822494 (-) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6S27_RS19335 (V6S27_19335) ampD 3822582..3823133 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6S27_RS19340 (V6S27_19340) ampE 3823130..3823984 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  V6S27_RS19345 (V6S27_19345) aroP 3824027..3825400 (-) 1374 WP_023145664.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=852637 V6S27_RS19325 WP_000360904.1 3820958..3821398(-) (pilA) [Escherichia coli strain S1-CAM-04-C]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=852637 V6S27_RS19325 WP_000360904.1 3820958..3821398(-) (pilA) [Escherichia coli strain S1-CAM-04-C]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363