Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V6S30_RS09290 Genome accession   NZ_CP145694
Coordinates   1733270..1733710 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain S1-KEN-04-A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1728270..1738710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S30_RS09270 (V6S30_09270) aroP 1729268..1730641 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  V6S30_RS09275 (V6S30_09275) ampE 1730684..1731538 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  V6S30_RS09280 (V6S30_09280) ampD 1731535..1732086 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6S30_RS09285 (V6S30_09285) nadC 1732174..1733067 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6S30_RS09290 (V6S30_09290) pilA 1733270..1733710 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  V6S30_RS09295 (V6S30_09295) pilB 1733720..1735105 (+) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  V6S30_RS09300 (V6S30_09300) hofC 1735095..1736297 (+) 1203 WP_000157234.1 protein transport protein HofC -
  V6S30_RS09305 (V6S30_09305) guaC 1736332..1737375 (-) 1044 WP_001217338.1 GMP reductase -
  V6S30_RS09310 (V6S30_09310) - 1737531..1737575 (-) 45 WP_120795372.1 protein YacM -
  V6S30_RS09315 (V6S30_09315) coaE 1737600..1738220 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=852342 V6S30_RS09290 WP_000360904.1 1733270..1733710(+) (pilA) [Escherichia coli strain S1-KEN-04-A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=852342 V6S30_RS09290 WP_000360904.1 1733270..1733710(+) (pilA) [Escherichia coli strain S1-KEN-04-A]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363