Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V6S33_RS21385 Genome accession   NZ_CP145679
Coordinates   4350094..4350771 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain S1-NGR-02-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4345094..4355771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S33_RS21350 (V6S33_21350) gspG 4345574..4346011 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  V6S33_RS21355 (V6S33_21355) gspH 4346019..4346528 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  V6S33_RS21360 (V6S33_21360) gspI 4346525..4346902 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V6S33_RS21365 (V6S33_21365) gspJ 4346895..4347482 (+) 588 WP_000610633.1 type II secretion system minor pseudopilin GspJ -
  V6S33_RS21370 (V6S33_21370) gspK 4347475..4348458 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V6S33_RS21375 (V6S33_21375) gspL 4348473..4349636 (+) 1164 WP_001115235.1 type II secretion system protein GspL -
  V6S33_RS21380 (V6S33_21380) gspM 4349633..4350094 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  V6S33_RS21385 (V6S33_21385) pilD 4350094..4350771 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  V6S33_RS21390 (V6S33_21390) bfr 4350800..4351276 (-) 477 WP_000675504.1 bacterioferritin -
  V6S33_RS21395 (V6S33_21395) bfd 4351348..4351542 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V6S33_RS21400 (V6S33_21400) chiA 4351711..4354404 (-) 2694 WP_000773150.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=852128 V6S33_RS21385 WP_000178161.1 4350094..4350771(+) (pilD) [Escherichia coli strain S1-NGR-02-A]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=852128 V6S33_RS21385 WP_000178161.1 4350094..4350771(+) (pilD) [Escherichia coli strain S1-NGR-02-A]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378