Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QYH60_RS04355 Genome accession   NZ_CP129292
Coordinates   813921..815282 (+) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain KMGR2-43     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 808921..820282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYH60_RS04310 (QYH60_04310) infA 809509..809727 (+) 219 WP_003130554.1 translation initiation factor IF-1 -
  QYH60_RS04315 (QYH60_04315) rpmJ 809781..809897 (+) 117 WP_001808836.1 50S ribosomal protein L36 -
  QYH60_RS04320 (QYH60_04320) rpsM 809915..810280 (+) 366 WP_003130555.1 30S ribosomal protein S13 -
  QYH60_RS04325 (QYH60_04325) rpsK 810301..810684 (+) 384 WP_010906297.1 30S ribosomal protein S11 -
  QYH60_RS04330 (QYH60_04330) - 810732..811670 (+) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  QYH60_RS04335 (QYH60_04335) rplQ 811689..812069 (+) 381 WP_003130558.1 50S ribosomal protein L17 -
  QYH60_RS04345 (QYH60_04345) - 813003..813233 (+) 231 WP_074453832.1 hypothetical protein -
  QYH60_RS04350 (QYH60_04350) - 813532..813759 (+) 228 WP_023188599.1 heavy-metal-associated domain-containing protein -
  QYH60_RS04355 (QYH60_04355) radA 813921..815282 (+) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  QYH60_RS04360 (QYH60_04360) - 815325..816011 (-) 687 WP_363325801.1 YoaK family protein -
  QYH60_RS04365 (QYH60_04365) - 816242..817327 (+) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  QYH60_RS04370 (QYH60_04370) - 817392..818021 (+) 630 WP_301401442.1 pyridoxamine 5'-phosphate oxidase family protein -
  QYH60_RS04375 (QYH60_04375) - 818142..818504 (+) 363 WP_003129889.1 PadR family transcriptional regulator -
  QYH60_RS04380 (QYH60_04380) - 818491..818838 (+) 348 WP_058207950.1 DUF1048 domain-containing protein -
  QYH60_RS04385 (QYH60_04385) - 818825..819148 (+) 324 WP_003129893.1 DUF1048 domain-containing protein -
  QYH60_RS04390 (QYH60_04390) - 819240..819734 (-) 495 WP_012898594.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=852088 QYH60_RS04355 WP_003130563.1 813921..815282(+) (radA) [Lactococcus lactis subsp. lactis strain KMGR2-43]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=852088 QYH60_RS04355 WP_003130563.1 813921..815282(+) (radA) [Lactococcus lactis subsp. lactis strain KMGR2-43]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCTTTGGTTCTGATTGGGGGAGATCCTGGGATAGGTAAATCAACTTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGGCCGAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTAACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCGATTG
TTTGTGCACTTGAGGGAACGCGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGACTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTGACAACTTTAACTGAATGTTTAAAACTGGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62