Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUE31_RS18555 Genome accession   NZ_AP027254
Coordinates   3705663..3706340 (+) Length   225 a.a.
NCBI ID   WP_000178169.1    Uniprot ID   -
Organism   Escherichia coli strain JNE120442     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3700663..3711340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE31_RS18520 (JNE120442_35220) gspG 3701143..3701580 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  QUE31_RS18525 (JNE120442_35230) gspH 3701588..3702097 (+) 510 WP_001076031.1 type II secretion system minor pseudopilin GspH -
  QUE31_RS18530 (JNE120442_35240) gspI 3702094..3702471 (+) 378 WP_001041738.1 type II secretion system minor pseudopilin GspI -
  QUE31_RS18535 (JNE120442_35250) gspJ 3702464..3703051 (+) 588 WP_000610629.1 type II secretion system minor pseudopilin GspJ -
  QUE31_RS18540 (JNE120442_35260) gspK 3703044..3704027 (+) 984 WP_001058528.1 type II secretion system minor pseudopilin GspK -
  QUE31_RS18545 (JNE120442_35270) gspL 3704042..3705205 (+) 1164 WP_001115245.1 type II secretion system protein GspL -
  QUE31_RS18550 (JNE120442_35280) gspM 3705202..3705663 (+) 462 WP_042107348.1 type II secretion system protein GspM -
  QUE31_RS18555 (JNE120442_35290) pilD 3705663..3706340 (+) 678 WP_000178169.1 prepilin peptidase GspO Machinery gene
  QUE31_RS18560 (JNE120442_35300) bfr 3706369..3706845 (-) 477 WP_000675505.1 bacterioferritin -
  QUE31_RS18565 (JNE120442_35310) bfd 3706917..3707111 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUE31_RS18570 (JNE120442_35320) chiA 3707280..3709982 (-) 2703 WP_000773176.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24812.67 Da        Isoelectric Point: 7.9585

>NTDB_id=85181 QUE31_RS18555 WP_000178169.1 3705663..3706340(+) (pilD) [Escherichia coli strain JNE120442]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=85181 QUE31_RS18555 WP_000178169.1 3705663..3706340(+) (pilD) [Escherichia coli strain JNE120442]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTAGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTAATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCGTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382

Multiple sequence alignment