Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QWP09_RS01945 Genome accession   NZ_CP129213
Coordinates   404280..405761 (-) Length   493 a.a.
NCBI ID   WP_011614486.1    Uniprot ID   P28615
Organism   Cupriavidus necator strain ALE26     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399280..410761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWP09_RS01915 (QWP09_01915) - 399598..400257 (-) 660 WP_010814381.1 LON peptidase substrate-binding domain-containing protein -
  QWP09_RS01920 (QWP09_01920) rapZ 400349..401233 (-) 885 WP_010814382.1 RNase adapter RapZ -
  QWP09_RS01925 (QWP09_01925) - 401352..401753 (-) 402 WP_011614484.1 PsiF family protein -
  QWP09_RS01930 (QWP09_01930) hprK 401916..402887 (-) 972 WP_010814384.1 HPr(Ser) kinase/phosphatase -
  QWP09_RS01935 (QWP09_01935) ptsN 402969..403424 (-) 456 WP_010814385.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QWP09_RS01940 (QWP09_01940) raiA 403881..404231 (-) 351 WP_010814386.1 ribosome-associated translation inhibitor RaiA -
  QWP09_RS01945 (QWP09_01945) rpoN/rpoN1 404280..405761 (-) 1482 WP_011614486.1 RNA polymerase factor sigma-54 Machinery gene
  QWP09_RS01950 (QWP09_01950) lptB 405896..406696 (-) 801 WP_010814388.1 LPS export ABC transporter ATP-binding protein -
  QWP09_RS01955 (QWP09_01955) lptA 406765..407415 (-) 651 WP_011614488.1 lipopolysaccharide transport periplasmic protein LptA -
  QWP09_RS01960 (QWP09_01960) lptC 407483..408112 (-) 630 WP_010814390.1 LPS export ABC transporter periplasmic protein LptC -
  QWP09_RS01965 (QWP09_01965) - 408156..409139 (-) 984 WP_010814391.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54770.07 Da        Isoelectric Point: 4.9195

>NTDB_id=851574 QWP09_RS01945 WP_011614486.1 404280..405761(-) (rpoN/rpoN1) [Cupriavidus necator strain ALE26]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEARADGAADDDSYGDSGNGDDYGSSDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISARDKGLAI
FLIESLDDDGYLSASLEEICTELPEELEFEIEEVHAILTLLQSFDPPGVGARNAAECLALQLRRLTHPQRELALNIVTNH
LELLAVRDYTRLKKALQVDEAALKSAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKGGGGWIAQLNPDVMPRLRINDMY
AQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDKILRVSQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=851574 QWP09_RS01945 WP_011614486.1 404280..405761(-) (rpoN/rpoN1) [Cupriavidus necator strain ALE26]
ATGAAACCGTCGCTACAGCTCCGCCTTTCCCAGCATCTGGCCCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTGGAACTGCAGCAAGAGGTCGAACAGGCACTGACGGAAAACCCTCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCGGCCGACGGCTCGGTCAACCTGCAGAGCGCACCGGCGCCCGCGCCGGCAGAGCCGCAG
GGCAATGGCGAGGCCCGCGCCGACGGCGCGGCTGACGACGACAGCTATGGCGACAGCGGCAACGGCGACGACTATGGCAG
CAGCGACTGGAGCCTGGACGACTTTGCCCGCCGCCCCCAGGGCGACGAGGACGAAAAAACGCCGATGCAGCTGCGCGAAG
CCGAGCCCACGCTGCGCGAGTACCTGATGGAACAGCTCACGCCGCTGAAGATCTCGGCGCGCGACAAGGGCCTGGCCATC
TTCCTGATCGAATCGCTCGACGACGATGGCTACCTGAGCGCATCGCTTGAGGAGATCTGCACGGAGTTGCCGGAAGAACT
CGAGTTCGAGATCGAGGAGGTCCACGCCATCCTCACGCTGCTGCAGAGCTTCGACCCGCCCGGCGTGGGCGCGCGCAACG
CCGCCGAGTGCCTGGCCCTGCAGTTGCGGCGCCTGACGCACCCGCAGCGCGAACTGGCCCTGAACATCGTGACCAACCAC
CTGGAGTTGCTCGCAGTACGCGACTACACGCGGCTGAAGAAGGCGCTGCAGGTGGATGAAGCGGCGCTGAAGTCCGCACA
CGAACTGATCCGCTCGCTGGCGCCCTACCCCGGCCATGCATACAGCCGCCCGGAGGCGGACTTCGTGGTGCCGGACGTGT
TCGTGCGCAAGGGTGGCGGCGGCTGGATCGCGCAGCTCAATCCGGATGTGATGCCGAGGCTGCGCATCAATGACATGTAT
GCGCAAATCCTACGTGGCGCAAAGGGTGAGTCCGGCACCGCCGGGCTGCAGCAGAAGCTGCAAGAGGCGCGCTGGCTGAT
CAAGAACATCCAGCAGAGGTTCGACAAAATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCAGCC
ACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCAACCATCTCCCGG
GTGACGACCAATAAATATATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCGTCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCC
TTTCCGACAGTCGCATTGCCGAACTGCTGGGCGAACAAGGCTTCGTTGTCGCACGCCGCACCGTTGCCAAGTATCGCGAA
GCCCTGAAGATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P28615

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.242

100

0.805