Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V6S45_RS18835 Genome accession   NZ_CP145630
Coordinates   3913872..3915305 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain S1-SRL-01-C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3908872..3920305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S45_RS18805 (V6S45_18805) yrbG 3909430..3910407 (+) 978 WP_000922865.1 calcium/sodium antiporter -
  V6S45_RS18810 (V6S45_18810) kdsD 3910421..3911407 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  V6S45_RS18815 (V6S45_18815) kdsC 3911428..3911994 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V6S45_RS18820 (V6S45_18820) lptC 3911991..3912566 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  V6S45_RS18825 (V6S45_18825) lptA 3912535..3913092 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V6S45_RS18830 (V6S45_18830) lptB 3913099..3913824 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  V6S45_RS18835 (V6S45_18835) rpoN/rpoN1 3913872..3915305 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  V6S45_RS18840 (V6S45_18840) hpf 3915328..3915615 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  V6S45_RS18845 (V6S45_18845) ptsN 3915733..3916224 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V6S45_RS18850 (V6S45_18850) rapZ 3916270..3917124 (+) 855 WP_000243741.1 RNase adapter RapZ -
  V6S45_RS18855 (V6S45_18855) npr 3917121..3917393 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  V6S45_RS18860 (V6S45_18860) yrbL 3917606..3918238 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  V6S45_RS18865 (V6S45_18865) mtgA 3918235..3918963 (-) 729 WP_000047061.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V6S45_RS18870 (V6S45_18870) elbB 3918960..3919613 (-) 654 WP_001469263.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=851287 V6S45_RS18835 WP_000809051.1 3913872..3915305(+) (rpoN/rpoN1) [Escherichia coli strain S1-SRL-01-C]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=851287 V6S45_RS18835 WP_000809051.1 3913872..3915305(+) (rpoN/rpoN1) [Escherichia coli strain S1-SRL-01-C]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CTGACACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCAGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGG
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465