Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   QWI19_RS12805 Genome accession   NZ_CP129123
Coordinates   2479710..2480120 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain 6D1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2475076..2500719 2479710..2480120 within 0


Gene organization within MGE regions


Location: 2475076..2500719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI19_RS12780 yqeF 2475243..2475974 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  QWI19_RS12785 cwlH 2476226..2476978 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QWI19_RS12790 yqeD 2477165..2477791 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  QWI19_RS12795 gnd 2477810..2478703 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QWI19_RS12800 yqeB 2478955..2479677 (+) 723 WP_010886572.1 hypothetical protein -
  QWI19_RS12805 nucA/comI 2479710..2480120 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QWI19_RS12810 - 2480316..2480666 (+) 351 Protein_2476 sigma-70 family RNA polymerase sigma factor -
  QWI19_RS12815 - 2480806..2481276 (+) 471 WP_015251668.1 MarR family transcriptional regulator -
  QWI19_RS12820 - 2481426..2482838 (+) 1413 WP_015251667.1 MDR family MFS transporter -
  QWI19_RS12825 spoIVCA 2483025..2484485 (-) 1461 WP_235701260.1 site-specific DNA recombinase SpoIVCA -
  QWI19_RS12830 - 2484443..2484621 (-) 179 Protein_2480 hypothetical protein -
  QWI19_RS12835 - 2485265..2486362 (-) 1098 WP_032726210.1 glycosyl hydrolase -
  QWI19_RS12845 - 2487295..2488422 (-) 1128 WP_015251664.1 Rap family tetratricopeptide repeat protein -
  QWI19_RS12855 - 2489202..2489876 (+) 675 WP_015251663.1 TraX family protein -
  QWI19_RS12860 - 2490015..2490149 (+) 135 WP_015251662.1 hypothetical protein -
  QWI19_RS12865 - 2490260..2490419 (+) 160 Protein_2485 hypothetical protein -
  QWI19_RS12870 - 2490789..2492555 (+) 1767 WP_015251660.1 T7SS effector LXG polymorphic toxin -
  QWI19_RS12875 - 2492569..2493033 (+) 465 WP_015251659.1 SMI1/KNR4 family protein -
  QWI19_RS12880 - 2493236..2493958 (-) 723 WP_015251658.1 NPP1 family protein -
  QWI19_RS12885 - 2494556..2495119 (-) 564 WP_015251657.1 TetR/AcrR family transcriptional regulator -
  QWI19_RS12890 - 2495317..2496204 (+) 888 WP_015251656.1 NmrA family NAD(P)-binding protein -
  QWI19_RS12895 - 2496254..2497222 (-) 969 WP_015251655.1 N-acetylmuramoyl-L-alanine amidase -
  QWI19_RS12900 - 2497267..2497407 (-) 141 Protein_2492 phage holin family protein -
  QWI19_RS12905 - 2497434..2497619 (-) 186 Protein_2493 phage terminase large subunit -
  QWI19_RS12910 terS 2497616..2498386 (-) 771 Protein_2494 phage terminase small subunit -
  QWI19_RS12915 - 2498568..2498654 (+) 87 WP_073991391.1 YjcZ family sporulation protein -
  QWI19_RS12920 - 2498695..2498787 (+) 93 WP_080019512.1 YjcZ family sporulation protein -
  QWI19_RS12925 - 2498973..2499188 (-) 216 WP_080019511.1 CotD family spore coat protein -
  QWI19_RS12930 yqaQ 2499746..2500155 (-) 410 Protein_2498 sigma-70 family RNA polymerase sigma factor -
  QWI19_RS12935 - 2500453..2500719 (+) 267 WP_029318115.1 hypothetical protein -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=851219 QWI19_RS12805 WP_009967785.1 2479710..2480120(-) (nucA/comI) [Bacillus subtilis strain 6D1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=851219 QWI19_RS12805 WP_009967785.1 2479710..2480120(-) (nucA/comI) [Bacillus subtilis strain 6D1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529