Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FLY89_RS02545 Genome accession   NZ_AP024415
Coordinates   533966..534766 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain NIPH17_0019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528966..539766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLY89_RS02525 (KHAB170019_04870) - 530067..531365 (+) 1299 WP_086225874.1 glycosyltransferase family 4 protein -
  FLY89_RS02530 (KHAB170019_04880) - 531390..531725 (+) 336 Protein_485 phosphatidylglycerophosphatase -
  FLY89_RS02535 (KHAB170019_04890) ndk 532164..532595 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  FLY89_RS02540 (KHAB170019_04900) rlmN 532718..533950 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FLY89_RS02545 (KHAB170019_04910) pilF 533966..534766 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FLY89_RS02550 (KHAB170019_04920) - 534757..535548 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  FLY89_RS02555 (KHAB170019_04930) ispG 535570..536685 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FLY89_RS02560 (KHAB170019_04940) hisS 536682..537974 (+) 1293 WP_000095261.1 histidine--tRNA ligase -
  FLY89_RS02565 (KHAB170019_04950) - 538003..538701 (+) 699 WP_000058787.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=85113 FLY89_RS02545 WP_004738086.1 533966..534766(+) (pilF) [Acinetobacter baumannii strain NIPH17_0019]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=85113 FLY89_RS02545 WP_004738086.1 533966..534766(+) (pilF) [Acinetobacter baumannii strain NIPH17_0019]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCTTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACTTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment