Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LC048_RS05420 Genome accession   NZ_CP129019
Coordinates   1093925..1095043 (+) Length   372 a.a.
NCBI ID   WP_306049652.1    Uniprot ID   -
Organism   Mesobacillus subterraneus strain KcN21-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1088925..1100043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC048_RS05380 (LC048_05380) - 1089083..1089256 (-) 174 WP_226602045.1 DUF2759 domain-containing protein -
  LC048_RS05385 (LC048_05385) - 1089270..1089851 (-) 582 WP_306049649.1 hypothetical protein -
  LC048_RS05390 (LC048_05390) - 1090025..1090660 (+) 636 WP_226602047.1 MBL fold metallo-hydrolase -
  LC048_RS24930 - 1090987..1091595 (+) 609 WP_371932001.1 SAM-dependent methyltransferase -
  LC048_RS24935 - 1091615..1091782 (+) 168 WP_371932002.1 SAM-dependent methyltransferase -
  LC048_RS24940 - 1091772..1092080 (+) 309 WP_371932003.1 SAM-dependent methyltransferase -
  LC048_RS05400 (LC048_05400) - 1092148..1092393 (-) 246 WP_226602050.1 DUF2626 domain-containing protein -
  LC048_RS05405 (LC048_05405) - 1092473..1093174 (-) 702 WP_226602051.1 metalloregulator ArsR/SmtB family transcription factor -
  LC048_RS05410 (LC048_05410) - 1093367..1093720 (+) 354 WP_226602052.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LC048_RS05420 (LC048_05420) comGA 1093925..1095043 (+) 1119 WP_306049652.1 competence type IV pilus ATPase ComGA Machinery gene
  LC048_RS05425 (LC048_05425) comGB 1095030..1096061 (+) 1032 WP_306049655.1 competence type IV pilus assembly protein ComGB -
  LC048_RS05430 (LC048_05430) comGC 1096100..1096417 (+) 318 WP_226602054.1 competence type IV pilus major pilin ComGC -
  LC048_RS05435 (LC048_05435) comGD 1096417..1096857 (+) 441 WP_306050447.1 competence type IV pilus minor pilin ComGD -
  LC048_RS05440 (LC048_05440) - 1096841..1097173 (+) 333 WP_226602056.1 hypothetical protein -
  LC048_RS05445 (LC048_05445) comGF 1097127..1097600 (+) 474 WP_226602057.1 competence type IV pilus minor pilin ComGF -
  LC048_RS05450 (LC048_05450) comGG 1097630..1097971 (+) 342 WP_306050450.1 competence type IV pilus minor pilin ComGG -
  LC048_RS05455 (LC048_05455) - 1097987..1098487 (+) 501 WP_226602058.1 shikimate kinase -
  LC048_RS05460 (LC048_05460) - 1098529..1098714 (+) 186 WP_226602059.1 YqzE family protein -
  LC048_RS05465 (LC048_05465) - 1098845..1098982 (-) 138 WP_226602060.1 hypothetical protein -
  LC048_RS05470 (LC048_05470) - 1099152..1099940 (-) 789 WP_226602061.1 YqhG family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 42020.38 Da        Isoelectric Point: 7.9725

>NTDB_id=850587 LC048_RS05420 WP_306049652.1 1093925..1095043(+) (comGA) [Mesobacillus subterraneus strain KcN21-5]
MYDEIFLYIRKRWCILIVKSIEQLADNVLKDAFRSGASDIHIIPREKDTLIKFRLGNQLLPRYTLDQADCERLISHFKFT
ASMDIGEKRRPQSGAFTYQYQEMKIGLRISTLPAYQSESMVIRLLPEQNQTPSYQISLFPSTSKKLISLLKHAHGLIIFT
GPTGSGKTTTLYSMLSETSDLVNRNVITLEDPIEKPSDTVLQVQVNERAGISYSAGLKAILRHDPDIIMVGEIRDKETAE
TAIRAALTGHLVLTTMHTRDARGAIYRLIEFGINWLEIEQTLIAVTAQRLVELTCPYCEGTCSPFCYSSGSGKRGNVFEI
LTGKDLSAVLKEARGELQSYHYKTLKDAIRKGIALGFIKESEYQRWVLNDGE

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=850587 LC048_RS05420 WP_306049652.1 1093925..1095043(+) (comGA) [Mesobacillus subterraneus strain KcN21-5]
GTGTATGACGAAATCTTTCTCTATATCCGAAAAAGGTGGTGCATCCTTATTGTTAAATCAATCGAACAGCTAGCGGATAA
TGTTTTGAAAGATGCTTTTAGAAGTGGCGCGTCGGACATTCATATTATCCCCCGTGAAAAAGACACACTGATTAAATTCA
GGCTCGGTAATCAGCTGCTTCCCCGGTACACGCTTGACCAGGCTGATTGTGAACGGCTCATATCCCACTTCAAATTTACA
GCATCAATGGATATTGGTGAAAAAAGACGGCCTCAAAGTGGTGCGTTTACTTATCAATATCAAGAAATGAAGATTGGCTT
AAGGATCTCTACGCTTCCAGCCTACCAGAGTGAAAGCATGGTCATCCGTCTCCTTCCCGAACAAAACCAAACTCCTTCAT
ACCAAATTTCATTATTCCCATCCACATCAAAAAAACTGATTTCCCTACTTAAACACGCACATGGTTTGATTATTTTTACT
GGCCCGACAGGCAGTGGCAAGACGACAACTTTATATTCGATGCTTTCTGAAACCTCGGATTTGGTGAATCGTAATGTCAT
TACGCTAGAAGATCCAATCGAAAAGCCGAGTGACACAGTGCTGCAGGTCCAGGTCAATGAACGCGCCGGAATATCATATT
CTGCCGGACTGAAAGCAATCCTCCGGCATGACCCGGATATTATAATGGTTGGGGAAATTCGGGATAAAGAGACTGCTGAA
ACGGCGATAAGAGCAGCGCTCACAGGACATCTTGTCCTGACAACGATGCATACAAGGGATGCCAGAGGAGCGATTTACAG
GCTGATTGAATTCGGGATTAATTGGCTTGAGATTGAACAAACATTGATCGCAGTTACGGCACAGAGACTCGTTGAGCTGA
CTTGCCCTTACTGCGAAGGTACATGTTCGCCATTTTGCTATTCATCAGGCAGCGGCAAAAGAGGAAATGTTTTTGAAATC
CTTACCGGGAAGGATTTATCTGCAGTACTGAAGGAAGCAAGAGGGGAACTCCAGAGCTATCATTATAAAACTCTCAAAGA
TGCAATCCGTAAAGGCATCGCGCTGGGGTTCATCAAGGAATCAGAATACCAGAGGTGGGTTCTCAATGATGGAGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(28-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.932

95.161

0.532
  pilB Glaesserella parasuis strain SC1401

38.395

93.817

0.36