Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QRA04_RS07405 Genome accession   NZ_CP128608
Coordinates   1392559..1392993 (+) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus tropicus strain PUMB_55     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1387559..1397993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRA04_RS07385 (QRA04_07365) - 1388454..1389611 (-) 1158 WP_000434566.1 aminotransferase A -
  QRA04_RS07390 (QRA04_07370) - 1389735..1389935 (-) 201 WP_000929285.1 hypothetical protein -
  QRA04_RS07395 (QRA04_07375) kinB 1390245..1391519 (+) 1275 WP_410490801.1 sporulation sensor histidine kinase KinB -
  QRA04_RS07400 (QRA04_07380) - 1391573..1392322 (-) 750 WP_196472502.1 DUF3967 domain-containing protein -
  QRA04_RS07405 (QRA04_07385) nucA/comI 1392559..1392993 (+) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  QRA04_RS07410 (QRA04_07390) comJ 1393030..1393419 (+) 390 WP_000424012.1 competence protein ComJ -
  QRA04_RS07415 (QRA04_07395) metE 1393457..1395745 (-) 2289 WP_410490802.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  QRA04_RS07420 (QRA04_07400) - 1396667..1397224 (-) 558 WP_226640753.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=849283 QRA04_RS07405 WP_000811502.1 1392559..1392993(+) (nucA/comI) [Bacillus tropicus strain PUMB_55]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=849283 QRA04_RS07405 WP_000811502.1 1392559..1392993(+) (nucA/comI) [Bacillus tropicus strain PUMB_55]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCATATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTGTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCATTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACACATTGAATATATAAGTCCAGCAGATAACCGTGGAGCAGGGTCTTGGGTAGGAAATAAGCTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549