Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QRA04_RS06175 Genome accession   NZ_CP128608
Coordinates   1185931..1186974 (+) Length   347 a.a.
NCBI ID   WP_410490769.1    Uniprot ID   -
Organism   Bacillus tropicus strain PUMB_55     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1180931..1191974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRA04_RS06140 (QRA04_06125) - 1181186..1181674 (+) 489 WP_042875547.1 hypothetical protein -
  QRA04_RS06145 (QRA04_06130) - 1181710..1182021 (-) 312 WP_001093243.1 hypothetical protein -
  QRA04_RS06150 (QRA04_06135) - 1182274..1182489 (+) 216 WP_001008320.1 DUF3912 family protein -
  QRA04_RS06155 (QRA04_06140) - 1182577..1182984 (+) 408 WP_242274917.1 hypothetical protein -
  QRA04_RS06160 (QRA04_06145) - 1183161..1184555 (-) 1395 WP_001094339.1 L-cystine transporter -
  QRA04_RS06165 (QRA04_06150) - 1184663..1184905 (-) 243 WP_000440717.1 DUF2626 domain-containing protein -
  QRA04_RS06170 (QRA04_06155) - 1185031..1185726 (-) 696 WP_000434132.1 helix-turn-helix transcriptional regulator -
  QRA04_RS06175 (QRA04_06160) comGA 1185931..1186974 (+) 1044 WP_410490769.1 competence protein ComGA Machinery gene
  QRA04_RS06180 (QRA04_06165) comGB 1186967..1187998 (+) 1032 WP_410490770.1 competence type IV pilus assembly protein ComGB -
  QRA04_RS06185 (QRA04_06170) comGC 1188010..1188309 (+) 300 WP_002194509.1 comG operon protein ComGC -
  QRA04_RS06190 (QRA04_06175) comGD 1188306..1188761 (+) 456 WP_196471537.1 comG operon protein ComGD -
  QRA04_RS06195 (QRA04_06180) comGE 1188754..1189056 (+) 303 WP_048550802.1 competence type IV pilus minor pilin ComGE -
  QRA04_RS06200 (QRA04_06185) comGF 1189026..1189496 (+) 471 WP_029439915.1 competence type IV pilus minor pilin ComGF -
  QRA04_RS06205 (QRA04_06190) comGG 1189493..1189864 (+) 372 WP_410490771.1 competence type IV pilus minor pilin ComGG -
  QRA04_RS06210 (QRA04_06195) - 1190040..1190690 (+) 651 WP_410490772.1 2OG-Fe(II) oxygenase -
  QRA04_RS06215 (QRA04_06200) aroK 1190809..1191306 (+) 498 WP_252210762.1 shikimate kinase AroK -
  QRA04_RS06220 (QRA04_06205) - 1191345..1191545 (+) 201 WP_000106081.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39316.78 Da        Isoelectric Point: 8.9053

>NTDB_id=849280 QRA04_RS06175 WP_410490769.1 1185931..1186974(+) (comGA) [Bacillus tropicus strain PUMB_55]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRSDDLLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=849280 QRA04_RS06175 WP_410490769.1 1185931..1186974(+) (comGA) [Bacillus tropicus strain PUMB_55]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGACAGAAGGATGTAGTGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTACGTCTTTCCACGCTTCCAACTGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGGTTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCGTTACACTGGAGGATCCAGTTGAAAAAAGAAGTGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGTCATTTAGTAATGACGACACTGCAT
ACAAATGATGCGAGAGGGGCGATACTCAGGTTCATGGATTTTGGCATAACGAGGCAAGAGATTGAACAATCTTTATTAGC
TATAGCTGCACAGAGACTTGTCGAATTAAAGTGTCCGTTTTGTAAAAGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAACACGAAACATTAGAATCTTCGATACGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366