Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BLKGLAD_RS18560 Genome accession   NZ_AP024337
Coordinates   4155190..4156377 (+) Length   395 a.a.
NCBI ID   WP_186027605.1    Uniprot ID   -
Organism   Burkholderia gladioli pv. gladioli strain MAFF 302385     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4150190..4161377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLKGLAD_RS18530 (BLKGLAD_35300) - 4150808..4151422 (+) 615 WP_013699419.1 50S ribosomal protein L25/general stress protein Ctc -
  BLKGLAD_RS18535 (BLKGLAD_35310) pth 4151519..4152124 (+) 606 WP_025097010.1 aminoacyl-tRNA hydrolase -
  BLKGLAD_RS18540 (BLKGLAD_35320) hisC 4152220..4153287 (+) 1068 WP_407852854.1 histidinol-phosphate transaminase -
  BLKGLAD_RS18545 (BLKGLAD_35330) - 4153434..4153706 (-) 273 WP_013699422.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BLKGLAD_RS18550 (BLKGLAD_35340) coaD 4153773..4154273 (-) 501 WP_013699423.1 pantetheine-phosphate adenylyltransferase -
  BLKGLAD_RS18555 (BLKGLAD_35350) rsmD 4154385..4154999 (-) 615 WP_407852855.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BLKGLAD_RS18560 (BLKGLAD_35360) pilA 4155190..4156377 (+) 1188 WP_186027605.1 signal recognition particle-docking protein FtsY Machinery gene
  BLKGLAD_RS18565 (BLKGLAD_35370) maiA 4156450..4157094 (-) 645 WP_046579733.1 maleylacetoacetate isomerase -
  BLKGLAD_RS18570 (BLKGLAD_35380) ybiB 4157308..4158288 (+) 981 WP_186162408.1 DNA-binding protein YbiB -
  BLKGLAD_RS18575 (BLKGLAD_35390) leuA 4158789..4160441 (+) 1653 WP_103690648.1 2-isopropylmalate synthase -
  BLKGLAD_RS18580 (BLKGLAD_35400) - 4160606..4160992 (-) 387 WP_013699429.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 41471.55 Da        Isoelectric Point: 4.7491

>NTDB_id=84876 BLKGLAD_RS18560 WP_186027605.1 4155190..4156377(+) (pilA) [Burkholderia gladioli pv. gladioli strain MAFF 302385]
MFSFFKRLIGSKADGAPTDDPDAPTDRQDAAEAPAAEAARPVERVAAPAPAPAVPVEPAAPAQVAPALPAAADEDEAQAV
TAVDVEIVPPPAPEPAAKKSWIARLRSGLSKTSSSLTSVFVSTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVKA
ERLTDPQQVKAALRGLLIALLAPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAARE
QLAIWGERNNVTVVQQESGDPAAVIFDAVGAARARKINVVMADTAGRLPTQLHLMEELRKVKRVIGKAQADAPHEVLLVI
DANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAEEFTDALLG

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=84876 BLKGLAD_RS18560 WP_186027605.1 4155190..4156377(+) (pilA) [Burkholderia gladioli pv. gladioli strain MAFF 302385]
ATGTTCAGCTTCTTCAAACGATTGATCGGCTCGAAGGCCGACGGCGCGCCGACCGACGACCCGGACGCGCCGACGGACCG
GCAGGACGCCGCCGAGGCTCCCGCGGCCGAAGCGGCCCGGCCCGTCGAGCGGGTCGCCGCGCCTGCCCCGGCCCCGGCGG
TTCCGGTCGAGCCGGCCGCGCCCGCGCAAGTCGCCCCTGCTCTCCCCGCCGCCGCGGACGAAGACGAAGCGCAGGCCGTC
ACGGCCGTCGACGTCGAGATCGTGCCGCCGCCCGCGCCGGAACCGGCCGCCAAGAAATCCTGGATCGCTCGCCTGCGCTC
GGGCCTGTCGAAGACCAGTTCCAGCCTGACGAGCGTCTTCGTCAGCACCAAGATCGACGAGGATCTCTACGAGGAGCTCG
AGACCGCGCTGCTGATGTCCGACGCCGGCGTCGACGCCACCGAGTACCTGCTCGGCGCGCTGCGCGAGAAGGTCAAGGCC
GAGCGGCTGACCGATCCGCAGCAGGTTAAGGCGGCCCTGCGCGGCCTGCTGATCGCGCTGCTCGCGCCGCTCGAGAAGTC
GCTGATGCTGGGCCGTGCGCAGCCGCTGGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCA
AGCTGGCCAAGCACCTGCAGAGCTTCGACCAGTCGGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGCGAG
CAGCTCGCTATCTGGGGCGAGCGCAACAACGTGACGGTGGTGCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGA
CGCGGTGGGCGCCGCGCGCGCGCGCAAGATCAACGTGGTGATGGCCGATACGGCGGGCCGCCTGCCCACCCAGCTGCACC
TGATGGAGGAACTGCGCAAGGTCAAGCGCGTGATCGGCAAGGCACAGGCCGACGCGCCGCACGAGGTGCTGCTGGTGATC
GACGCCAACACCGGTCAGAACGCGCTCGCCCAGGTAAAGGCCTTCGACGACGCGCTGGGCCTCACCGGCCTGATCGTCAC
CAAGCTCGACGGCACCGCGAAGGGCGGCATCCTCGCCGCGATCGCGCGGCAGCGGCCGGTACCGGTCTACTTCATCGGCG
TCGGCGAGAAGGTGGAGGACCTGCAGCCCTTCAATGCCGAGGAGTTCACCGACGCGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(196-394)

SRP54-type protein, helical bundle domain (SRP54_N)

(114-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

63.576

76.456

0.486

Multiple sequence alignment