Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BLKGLAD_RS18465 Genome accession   NZ_AP024337
Coordinates   4138468..4139973 (+) Length   501 a.a.
NCBI ID   WP_046577596.1    Uniprot ID   -
Organism   Burkholderia gladioli pv. gladioli strain MAFF 302385     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4133468..4144973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLKGLAD_RS18440 (BLKGLAD_35130) kdsD 4134677..4135660 (+) 984 WP_025096995.1 arabinose 5-phosphate isomerase KdsD -
  BLKGLAD_RS18445 (BLKGLAD_35140) - 4135660..4136199 (+) 540 WP_013699403.1 KdsC family phosphatase -
  BLKGLAD_RS18450 (BLKGLAD_35150) lptC 4136203..4136817 (+) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  BLKGLAD_RS18455 (BLKGLAD_35160) lptA 4136836..4137504 (+) 669 WP_105859100.1 lipopolysaccharide transport periplasmic protein LptA -
  BLKGLAD_RS18460 (BLKGLAD_35170) lptB 4137501..4138277 (+) 777 WP_407852852.1 LPS export ABC transporter ATP-binding protein -
  BLKGLAD_RS18465 (BLKGLAD_35180) rpoN/rpoN1 4138468..4139973 (+) 1506 WP_046577596.1 RNA polymerase factor sigma-54 Machinery gene
  BLKGLAD_RS18470 (BLKGLAD_35190) hpf 4140124..4140489 (+) 366 WP_013699408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BLKGLAD_RS18475 (BLKGLAD_35200) - 4140703..4141215 (+) 513 WP_025097001.1 PTS sugar transporter subunit IIA -
  BLKGLAD_RS18480 (BLKGLAD_35210) hprK 4141341..4142309 (+) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  BLKGLAD_RS18485 (BLKGLAD_35220) rapZ 4142420..4143313 (+) 894 WP_013699411.1 RNase adapter RapZ -
  BLKGLAD_RS18490 (BLKGLAD_35230) - 4143346..4143981 (+) 636 WP_013699412.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55103.08 Da        Isoelectric Point: 4.9483

>NTDB_id=84875 BLKGLAD_RS18465 WP_046577596.1 4138468..4139973(+) (rpoN/rpoN1) [Burkholderia gladioli pv. gladioli strain MAFF 302385]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEPSGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=84875 BLKGLAD_RS18465 WP_046577596.1 4138468..4139973(+) (rpoN/rpoN1) [Burkholderia gladioli pv. gladioli strain MAFF 302385]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCCTCCTCGACGCCCGAGCCGATGCACGGT
GGCGGCTCGAACGGCAGCGAACCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGTCGCTCCTCGGGCGCCTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAAGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCGGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGAGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACGACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCGCACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment