Detailed information NTG0848609

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LU687_RS03640
• Genome accession: NZ_CP128542
• Coordinates: 779509..780375 (-)
• Length: 288 a.a.
• NCBI ID: WP_015268863.1
• Uniprot ID: A0A2N5B7I0
• Organism: Pseudomonas asiatica strain NMI3658_15
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 774509..785375

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LU687_RS03605 (LU687_003605)	-	774723..776018 (-)	1296	WP_015268859.1	NAD(P)/FAD-dependent oxidoreductase	-
LU687_RS03610 (LU687_003610)	-	776103..776276 (-)	174	WP_013970765.1	DUF3094 family protein	-
LU687_RS03615 (LU687_003615)	-	776410..777039 (-)	630	WP_013970766.1	DUF1780 domain-containing protein	-
LU687_RS03620 (LU687_003620)	-	777095..777526 (-)	432	WP_015268860.1	hypothetical protein	-
LU687_RS03625 (LU687_003625)	-	777613..778320 (+)	708	WP_043935298.1	energy-coupling factor ABC transporter permease	-
LU687_RS03630 (LU687_003630)	yacG	778689..778889 (-)	201	WP_003260624.1	DNA gyrase inhibitor YacG	-
LU687_RS03635 (LU687_003635)	coaE	778886..779509 (-)	624	WP_015268862.1	dephospho-CoA kinase	-
LU687_RS03640 (LU687_003640)	pilD	779509..780375 (-)	867	WP_015268863.1	A24 family peptidase	Machinery gene
LU687_RS03645 (LU687_003645)	-	780378..781586 (-)	1209	WP_015268864.1	type II secretion system F family protein	-
LU687_RS03650 (LU687_003650)	-	781802..782212 (+)	411	WP_015268865.1	pilin	-
LU687_RS03660 (LU687_003660)	-	782987..784273 (+)	1287	WP_158242119.1	ATP-binding protein	-
LU687_RS03665 (LU687_003665)	-	784275..785255 (+)	981	WP_015268866.1	hypothetical protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 32047.21 Da
• Isoelectric Point: 7.9596

>NTDB_id=848609 LU687_RS03640 WP_015268863.1 779509..780375(-) (pilD) [Pseudomonas asiatica strain NMI3658_15]
MTLWTLLAEQPAYFLTLATVLGLLVGSFLNVLVYRLPIMLERQWQREAQEMLELPTTPHERFDLWLPASRCPHCAHRIRA
WENIPVISYLALRGRCSACKTRISPRYPLVELASALLSLVVAWRFGASPEALLALPLTWCLLALSLIDAEHQLLPDALVL
PMLWLGLIVNAFGLYVPLTSALWGAVAGYLSLWTVYWVFKLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LAGLCLLRLRRHAMGTAIPFGPYLAIAGWIAVLWGDEIYVSYTNLLGF

Nucleotide

• Length: 867 bp

>NTDB_id=848609 LU687_RS03640 WP_015268863.1 779509..780375(-) (pilD) [Pseudomonas asiatica strain NMI3658_15]
ATGACTTTATGGACTTTGCTGGCTGAGCAGCCGGCGTACTTTCTCACCCTGGCCACGGTGCTCGGCCTGCTGGTGGGCAG
TTTTCTCAATGTGCTGGTGTACCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAGGAAATGCTGGAGT
TGCCGACCACGCCGCACGAGCGTTTCGACCTGTGGCTACCCGCCTCGCGCTGCCCGCATTGCGCGCATCGGATTCGCGCA
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGCGCGGGCGTTGCTCAGCCTGCAAAACCCGCATCAGCCCGCGCTA
CCCATTGGTGGAGTTAGCCAGCGCGCTGCTGTCGCTGGTGGTGGCCTGGCGCTTTGGTGCTTCGCCCGAGGCCTTGCTGG
CGCTGCCGCTGACCTGGTGCCTGCTGGCCCTGAGCCTGATCGATGCCGAGCACCAGTTGCTGCCGGATGCGCTGGTGTTG
CCGATGCTGTGGCTGGGGCTGATCGTCAACGCCTTTGGCCTCTACGTCCCGCTGACCAGTGCGTTGTGGGGCGCGGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAAGCTGGTTACCGGCAAGGAGGGCATGGGCTATGGCGACTTCA
AGCTGATGGCGCTGATCGGGGCCTGGGGTGGCTGGCAGGTGTTGCCGTTGACCCTGCTGCTGTCGTCGGTGGTCGGGGCG
CTGGCTGGGCTGTGCCTGTTGCGCTTGCGCCGCCATGCCATGGGCACGGCGATCCCGTTTGGCCCCTATCTGGCGATTGC
GGGGTGGATTGCAGTGCTCTGGGGTGATGAAATATACGTCTCATACACAAACCTGCTTGGATTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: A0A2N5B7I0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.982	94.097	0.517
pilD	Acinetobacter baumannii D1279779	52.143	97.222	0.507
pilD	Acinetobacter nosocomialis M2	51.429	97.222	0.5
pilD	Vibrio campbellii strain DS40M4	49.822	97.569	0.486
pilD	Neisseria gonorrhoeae MS11	50.929	93.403	0.476