Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V5100_RS20865 Genome accession   NZ_CP144930
Coordinates   4131802..4132242 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SCPM-O-B-9504     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4126802..4137242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5100_RS20845 (V5100_20855) aroP 4127800..4129173 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  V5100_RS20850 (V5100_20860) ampE 4129216..4130070 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  V5100_RS20855 (V5100_20865) ampD 4130067..4130618 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V5100_RS20860 (V5100_20870) nadC 4130706..4131599 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V5100_RS20865 (V5100_20875) pilA 4131802..4132242 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  V5100_RS20870 (V5100_20880) pilB 4132252..4133637 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  V5100_RS20875 (V5100_20885) hofC 4133627..4134829 (+) 1203 WP_000157266.1 protein transport protein HofC -
  V5100_RS20880 (V5100_20890) guaC 4134864..4135907 (-) 1044 WP_001217338.1 GMP reductase -
  V5100_RS20885 (V5100_20895) - 4136063..4136107 (-) 45 WP_120795372.1 protein YacM -
  V5100_RS20890 (V5100_20900) coaE 4136132..4136752 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=848435 V5100_RS20865 WP_000360904.1 4131802..4132242(+) (pilA) [Escherichia coli strain SCPM-O-B-9504]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=848435 V5100_RS20865 WP_000360904.1 4131802..4132242(+) (pilA) [Escherichia coli strain SCPM-O-B-9504]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCTAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363