Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V5100_RS02050 Genome accession   NZ_CP144930
Coordinates   434215..434892 (-) Length   225 a.a.
NCBI ID   WP_000178157.1    Uniprot ID   -
Organism   Escherichia coli strain SCPM-O-B-9504     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 429215..439892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5100_RS02025 (V5100_02015) bfd 430904..431098 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V5100_RS02030 (V5100_02020) - 431145..432758 (-) 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
  V5100_RS02035 (V5100_02025) tnpB 432789..433139 (-) 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
  V5100_RS02040 (V5100_02030) - 433136..433561 (-) 426 WP_000422741.1 transposase -
  V5100_RS02045 (V5100_02035) bfr 433710..434186 (+) 477 WP_000675504.1 bacterioferritin -
  V5100_RS02050 (V5100_02040) pilD 434215..434892 (-) 678 WP_000178157.1 prepilin peptidase GspO Machinery gene
  V5100_RS02055 (V5100_02045) gspM 434892..435353 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  V5100_RS02060 (V5100_02050) gspL 435350..436513 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  V5100_RS02065 (V5100_02055) gspK 436528..437511 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V5100_RS02070 (V5100_02060) gspJ 437504..438091 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V5100_RS02075 (V5100_02065) gspI 438084..438461 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V5100_RS02080 (V5100_02070) gspH 438458..438967 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  V5100_RS02085 (V5100_02075) gspG 438975..439412 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24932.80 Da        Isoelectric Point: 7.9563

>NTDB_id=848385 V5100_RS02050 WP_000178157.1 434215..434892(-) (pilD) [Escherichia coli strain SCPM-O-B-9504]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGFVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=848385 V5100_RS02050 WP_000178157.1 434215..434892(-) (pilD) [Escherichia coli strain SCPM-O-B-9504]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTTGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378