Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V5099_RS01980 Genome accession   NZ_CP144928
Coordinates   419698..420375 (-) Length   225 a.a.
NCBI ID   WP_000178157.1    Uniprot ID   -
Organism   Escherichia coli strain SCPM-O-B-12142     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414698..425375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5099_RS01965 (V5099_01965) chiA 416065..418758 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  V5099_RS01970 (V5099_01970) bfd 418927..419121 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V5099_RS01975 (V5099_01975) bfr 419193..419669 (+) 477 WP_000675504.1 bacterioferritin -
  V5099_RS01980 (V5099_01980) pilD 419698..420375 (-) 678 WP_000178157.1 prepilin peptidase GspO Machinery gene
  V5099_RS01985 (V5099_01985) gspM 420375..420836 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  V5099_RS01990 (V5099_01990) gspL 420833..421996 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  V5099_RS01995 (V5099_01995) gspK 422011..422994 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V5099_RS02000 (V5099_02000) gspJ 422987..423574 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V5099_RS02005 (V5099_02005) gspI 423567..423944 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V5099_RS02010 (V5099_02010) gspH 423941..424450 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  V5099_RS02015 (V5099_02015) gspG 424458..424895 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24932.80 Da        Isoelectric Point: 7.9563

>NTDB_id=848303 V5099_RS01980 WP_000178157.1 419698..420375(-) (pilD) [Escherichia coli strain SCPM-O-B-12142]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGFVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=848303 V5099_RS01980 WP_000178157.1 419698..420375(-) (pilD) [Escherichia coli strain SCPM-O-B-12142]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTTGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378