Detailed information NTG0848110

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QUC27_RS02075
• Genome accession: NZ_CP128507
• Coordinates: 444295..445443 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain WS27-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 439295..450443

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUC27_RS02050 (QUC27_02050)	-	440446..440742 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QUC27_RS02055 (QUC27_02055)	-	440739..441332 (-)	594	WP_003084544.1	YggT family protein	-
QUC27_RS02060 (QUC27_02060)	proC	441343..442164 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QUC27_RS02065 (QUC27_02065)	-	442176..442868 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QUC27_RS02070 (QUC27_02070)	pilT	443083..444117 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QUC27_RS02075 (QUC27_02075)	pilU	444295..445443 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QUC27_RS02080 (QUC27_02080)	czcD	445451..446350 (-)	900	WP_034028654.1	CDF family zinc transporter CzcD	-
QUC27_RS02085 (QUC27_02085)	-	446505..446909 (+)	405	WP_121295518.1	TM2 domain-containing protein	-
QUC27_RS02090 (QUC27_02090)	-	447152..448525 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QUC27_RS02095 (QUC27_02095)	-	448522..449706 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=848110 QUC27_RS02075 WP_003100959.1 444295..445443(+) (pilU) [Pseudomonas aeruginosa strain WS27-3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=848110 QUC27_RS02075 WP_003100959.1 444295..445443(+) (pilU) [Pseudomonas aeruginosa strain WS27-3]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382