Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NK8_RS01255 Genome accession   NZ_AP024322
Coordinates   251285..252802 (-) Length   505 a.a.
NCBI ID   WP_162064790.1    Uniprot ID   -
Organism   Caballeronia sp. NK8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 246285..257802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK8_RS01230 (NK8_02420) - 247064..247696 (-) 633 WP_213226893.1 LON peptidase substrate-binding domain-containing protein -
  NK8_RS01235 (NK8_02430) rapZ 247756..248628 (-) 873 WP_213226894.1 RNase adapter RapZ -
  NK8_RS01240 (NK8_02440) hprK 248711..249679 (-) 969 WP_061172712.1 HPr(Ser) kinase/phosphatase -
  NK8_RS01245 (NK8_02450) ptsN 249867..250358 (-) 492 WP_353622641.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NK8_RS01250 (NK8_02460) raiA 250741..251100 (-) 360 WP_162064789.1 ribosome-associated translation inhibitor RaiA -
  NK8_RS01255 (NK8_02470) rpoN/rpoN1 251285..252802 (-) 1518 WP_162064790.1 RNA polymerase factor sigma-54 Machinery gene
  NK8_RS01260 (NK8_02480) lptB 252954..253721 (-) 768 WP_174257975.1 LPS export ABC transporter ATP-binding protein -
  NK8_RS01265 (NK8_02490) lptA 253733..254419 (-) 687 WP_213226895.1 lipopolysaccharide transport periplasmic protein LptA -
  NK8_RS01270 (NK8_02500) lptC 254450..255052 (-) 603 WP_061172717.1 LPS export ABC transporter periplasmic protein LptC -
  NK8_RS01275 (NK8_02510) - 255057..255596 (-) 540 WP_061172718.1 HAD family hydrolase -
  NK8_RS01280 (NK8_02520) kdsD 255596..256579 (-) 984 WP_162064793.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55454.49 Da        Isoelectric Point: 4.8796

>NTDB_id=84794 NK8_RS01255 WP_162064790.1 251285..252802(-) (rpoN/rpoN1) [Caballeronia sp. NK8]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAIAQNPLLENEDDWIASPLRVAADGSLIASPATQNAPSESM
MSNGSSSSTSSSERSESSEPQGVDEYNGMGSDSGQDSSSWNLEDYGRSNNASDDDDLPPLQIHESTTTLRDHLTAQLRMT
AANQRDRALVTFLIESLDEDGYLTSSLDEVQMDLPEELEVDLDELSAALALLQSFDPPGVGGRSASECLKLQLLRLDGSA
TRTLALEIVTNHLELLAARDFTRLRKQLKAGDDALRDAHVLIKSLEPFPGAAYGKSEADYVVPDILVRKSASGWTAELNP
EIVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=84794 NK8_RS01255 WP_162064790.1 251285..252802(-) (rpoN/rpoN1) [Caballeronia sp. NK8]
ATGAAAGCCAGCCTCCAACTCCGCCTCTCGCAGCATCTTGCGCTGACCCCGCAACTTCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCATCGCCCAGAATCCGCTGCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGCTGCGCGTTGCCGCGGACGGATCGCTGATCGCATCGCCGGCGACGCAGAACGCGCCGTCCGAGTCGATG
ATGAGCAATGGCTCGTCGTCAAGCACGTCCAGTTCCGAACGTTCGGAAAGCAGCGAACCACAGGGCGTCGACGAATACAA
CGGCATGGGTTCGGACAGCGGCCAGGATTCGAGTTCATGGAATCTGGAAGACTACGGCCGCTCGAACAACGCCTCCGACG
ACGACGATCTCCCGCCGTTGCAAATCCACGAATCGACCACGACGCTGCGCGACCATCTGACGGCGCAGTTGCGCATGACC
GCGGCGAATCAGCGCGATCGCGCGCTCGTCACGTTTCTGATCGAATCGCTGGACGAAGACGGTTATCTGACTTCATCGCT
CGATGAGGTTCAGATGGATCTGCCCGAGGAACTCGAAGTCGATCTCGACGAACTCAGCGCAGCGCTTGCGTTGCTGCAAA
GTTTCGACCCGCCCGGTGTGGGCGGTCGCTCGGCATCGGAATGCCTGAAGCTGCAGTTGCTTCGGCTCGACGGCTCGGCC
ACGCGCACGCTCGCGCTCGAGATCGTCACGAACCATCTGGAACTGCTCGCCGCGCGTGACTTTACGCGCCTGCGCAAACA
ACTGAAGGCAGGCGACGACGCCCTGCGCGACGCGCATGTGCTGATCAAGTCGCTGGAGCCGTTTCCGGGCGCCGCGTACG
GAAAGAGCGAAGCGGATTACGTGGTGCCGGACATTCTCGTGCGCAAGAGCGCGAGCGGCTGGACGGCGGAGCTGAATCCG
GAGATCGTGCCGAAGCTGCGGATCAATCATCTGTACGCGAATATCCTGCGCAACAATCGTGGAGACCCTGGCAGCGGTTC
ACTGCGTCAGCAGCTTCAGGAAGCGCGCTGGCTCATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGTGTCGCGC
AGGCCATTGTGGAGCGTCAAAAGAACTTCTTTGCACATGGTGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAGATTGCT
GATACGCTGGGATTACACGAATCGACGGTTTCTCGTGTGACGACAGGGAAGTACATGCTCACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTTGGATCGCACGTTTCGACCGACACGGGAGGCGCGGCATCGTCCACGGCGATCCGGGCCCTCATCA
AGCAACTGATAGGAGCGGAAGACCCCAAAACTCCTCTTTCAGACAGCCGCATCGCCGAACTGCTGGCGGAACAAGGATTC
GTGGTCGCGCGGCGCACCGTCGCCAAGTACCGCGAAGCCCTCAAGATCCCTGCAGTGAATCTGCGCAAGTCTCTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

65.941

100

0.659

Multiple sequence alignment