Detailed information NTG0847713

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: V4U93_RS05000
• Genome accession: NZ_CP144755
• Coordinates: 991589..995323 (+)
• Length: 1244 a.a.
• NCBI ID: WP_226474277.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei subsp. sakei strain DCF0720
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 986589..1000323

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4U93_RS04990 (V4U93_04990)	mvk	986812..987759 (-)	948	WP_025015793.1	mevalonate kinase	-
V4U93_RS04995 (V4U93_04995)	-	988036..991596 (+)	3561	WP_016265071.1	PD-(D/E)XK nuclease family protein	-
V4U93_RS05000 (V4U93_05000)	addA	991589..995323 (+)	3735	WP_226474277.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
V4U93_RS05005 (V4U93_05005)	-	995389..998223 (+)	2835	WP_085391439.1	helicase C-terminal domain-containing protein	-
V4U93_RS05010 (V4U93_05010)	-	998301..998777 (+)	477	WP_011374615.1	DUF5590 domain-containing protein	-
V4U93_RS05015 (V4U93_05015)	asnS	998888..1000186 (+)	1299	WP_011374616.1	asparagine--tRNA ligase	-

Sequence

Protein

• Length: 1244 a.a.
• Molecular weight: 141274.03 Da
• Isoelectric Point: 4.7686

>NTDB_id=847713 V4U93_RS05000 WP_226474277.1 991589..995323(+) (addA) [Latilactobacillus sakei subsp. sakei strain DCF0720]
MSNPQFTPSQQAAVDHDGHDILVSASAGSGKTSVLVARVIQKILNGTDVDTLLVVTFTEAAATEMRQRIQVALRDASEKA
TEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYNHEYVKENEQ
DTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNNLPKSYAVDEAGLTASDYFQKKILPILQQTITECLEAL
QEAEQLSQTSENLMIYAPQIATTQAALTQINADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDDVKK
KIQTLLDTYFVFDEATTANIQRQAGQLVQKLVSVTLTFRAAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFYQQKF
SEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENFRSRR
NIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRIQAM
MTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQDIPL
VSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDLIWT
IYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIHGSK
GLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRAEQR
LIIVGHCDNQAQLMKQWQKGQNSTQQILDDGVRNDAASLLDWIGLSLIRHPQAKDWTTDYEPLLALSGDQTEFKLIMTNE
TLLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQLDLA
AEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQALVDRLVLQNSLSANVAAKINLNHL
LTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQEAA
IERVKTRYAGQINLYSAALNQIAQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3735 bp

>NTDB_id=847713 V4U93_RS05000 WP_226474277.1 991589..995323(+) (addA) [Latilactobacillus sakei subsp. sakei strain DCF0720]
TTGAGTAACCCCCAATTTACACCTTCACAGCAAGCCGCTGTCGATCATGATGGCCACGATATTTTAGTATCCGCATCAGC
TGGTTCAGGGAAGACATCCGTTCTTGTTGCGCGGGTCATCCAAAAAATCCTAAACGGGACCGATGTCGATACCTTACTTG
TTGTGACGTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGTAGCACTCCGCGACGCCAGTGAGAAAGCA
ACAGAACCAGCCGTTAAGCAACGCTTACGGCAACAATTAAGTCTCGTGCCTACCGCACAAATTAGTACACTGCATGCCTT
TTGTTTAAAAGTCATTAAACAATTTTATTATGTCATTGATCGTGATCCCGTATTCCGCTTATTGAGTGATACCGCAGAAC
GACTCCTATTAGCGGATCAAGTATGGCAACGGGTGAGAGAGGCCTTCTATAATCACGAATATGTCAAAGAAAATGAGCAA
GATACATTATTCTACGAATTAGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTGACCGACATTGTTTTTGAGTT
GCTCGATTTTGCAAACGCCAATCCAAACCCGGTTAAATGGTTAAATAACCTTCCCAAAAGTTATGCCGTTGACGAAGCTG
GTTTGACGGCCAGTGATTATTTCCAAAAAAAAATATTACCGATTTTACAGCAAACCATCACCGAATGTTTGGAAGCCTTA
CAAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAATGATCTATGCCCCTCAAATTGCAACTACGCAAGCAGCCTT
AACACAAATTAACGCAGATGCAAAAACGTTGAATTGGCAAGCATGGCGCCAACAACTAACAGATGCACAGCTGGGGCCTG
CTAAACGGACTAAGAAATTAGAACCCGACGAACAAATTAATAAGGACCGGTTGAAAGCCGATTTAGATGACGTCAAAAAG
AAAATTCAGACGTTACTGGACACCTATTTTGTGTTTGATGAAGCAACCACCGCTAATATTCAACGTCAAGCTGGCCAATT
GGTTCAAAAATTGGTGAGCGTCACGTTAACTTTTAGAGCAGCCTTTCAAGCCGAAAAAGAACGACGTCATTTATTGGACT
TTAGTGATTTAGAACAACTTTGTCTGACAATTTTATCGGCAGAGGATTCGCCGGCAAGGGCCTTTTACCAACAAAAATTT
AGCGAAGTGCTAGTTGATGAATATCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCACTTCTGATCATCC
ACGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTATTTAAGAATA
AGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGTTCGCGCCGC
AATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTCGGCGAGTTAGACTACGACGAAAATGC
GGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATGAAACTAAGC
CGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATTCAAGCCATG
ATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACATCGTTTTACTAGC
CCCAACCCGTGGTAATAATTTGTTTATCCTGGATTATTTCAAACGCTTCGGTTTACCGGTTGTGATTAAGGATGCGCAAA
ACTATTTCCAAACGACCGAAGTCCAAATTATGTTAGCTCTACTTCAGGTGATTGATAATCCTAACCAAGATATTCCATTG
GTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAAGACGGATGA
TTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAACGAGCAGAAAGTCGGGCATTTAGGCCAACAGGTCATGCAGA
AGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATTGCTCGCCAACAACCGATTGTGGACTTAATTTGGACG
ATCTACCAAGAAACTGGCTTTTTAGACTATGTCGGTGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTTACACGCGCT
TTATGAACGTGCCACGGCGTATGAAGAAAATGGCTTTAAAGGACTCTTCCAATTTATTCAATTTATTGAACGTCTCCAGA
AACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAGGACGCCATCTCTGTTATGACCATTCACGGGAGTAAG
GGGCTTGAATTTCCAGTCGTCTTTTTAATCGATACGAGTCGTCGCTTTAACCAACAGGATTTACAACGCTCATATGTTTT
GGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTGCCAACACTGCCAGAGTTAGCGA
TTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCGGAACAACGG
TTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTACCCAACAAAT
ATTAGATGATGGGGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATCCACAAGCTA
AAGATTGGACCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAATTTAAGTTGATTATGACTAATGAA
ACCTTACTTGGTGAAATACCAGGTAGTCTGCATAACGATCAGGACTGGGGACAACAACAGCAAAAGGCACTTCAAACAAT
TGATTTAAACAGTGGGATAGCAACTGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCCACCCAAACGA
CGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGGTTGGGACAATTAGACCTAGCG
GCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAACCGTTCAACAACC
AACGGCAGCAGAAGTAGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGCTAGCGAGTT
TGCAAGCCCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTTAATCACCTA
TTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCCATTTTCGTT
GTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGATCACGTCCTAATCCATGGGATTATGGATG
GTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGATCAAGAAGCGGCG
ATTGAACGGGTTAAAACCCGCTATGCTGGCCAGATTAACCTCTATAGTGCTGCTTTAAACCAGATTGCCCAAAAGCCAGT
GACCCATAAATATCTCTATTTATTGTCAATTGGCGAGTTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1064-1227)

• UvrD-like helicase C-terminal domain (UvrD_C) (470-884)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (7-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.51	100	0.383