Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QSJ10_RS00525 Genome accession   NZ_CP128494
Coordinates   99680..101050 (+) Length   456 a.a.
NCBI ID   WP_033017265.1    Uniprot ID   A0A150N5A2
Organism   Geobacillus stearothermophilus ATCC 12980     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 94680..106050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSJ10_RS00505 (QSJ10_00505) ctsR 95052..95513 (+) 462 WP_033017268.1 transcriptional regulator CtsR -
  QSJ10_RS00510 (QSJ10_00510) - 95528..96076 (+) 549 WP_033008611.1 UvrB/UvrC motif-containing protein -
  QSJ10_RS00515 (QSJ10_00515) - 96081..97172 (+) 1092 WP_287136169.1 protein arginine kinase -
  QSJ10_RS00520 (QSJ10_00520) clpC 97169..99604 (+) 2436 WP_033017266.1 ATP-dependent protease ATP-binding subunit ClpC -
  QSJ10_RS00525 (QSJ10_00525) radA 99680..101050 (+) 1371 WP_033017265.1 DNA repair protein RadA Machinery gene
  QSJ10_RS00530 (QSJ10_00530) - 101336..102430 (+) 1095 WP_033017264.1 PIN/TRAM domain-containing protein -
  QSJ10_RS00535 (QSJ10_00535) ispD 102452..103138 (+) 687 WP_033008621.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  QSJ10_RS00540 (QSJ10_00540) ispF 103153..103635 (+) 483 WP_033008623.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  QSJ10_RS00545 (QSJ10_00545) gltX 103742..105211 (+) 1470 WP_049624214.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49365.85 Da        Isoelectric Point: 7.7488

>NTDB_id=847678 QSJ10_RS00525 WP_033017265.1 99680..101050(+) (radA) [Geobacillus stearothermophilus ATCC 12980]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIEPVRTAVRGAFLHSEPSGSAKPVPITAVAAVQEPRIETNSAEF
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQASAQLAARHTVLYVSGEESVKQVKLRAGRLRAESDHLYVLAEANLEYIVS
AIETIQPSCVVIDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGVAIFIVGHVTKEGAIAGPRILEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSHGAAGSTVVAAMEGTRPVLVEIQALVSPTSFG
NPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDRPTNPTDVIIGEVGLTG
EVRRVSRIEQRVQEAVKLGFSRVIVPKNNLAGWQPPASVQVIGVSHVAEALEHTMI

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=847678 QSJ10_RS00525 WP_033017265.1 99680..101050(+) (radA) [Geobacillus stearothermophilus ATCC 12980]
ATGGCGAAAAAGAAGACGAAATTTGTTTGCCAAGAGTGCGGATACGAATCGGCGAAGTGGCTCGGCCGCTGCCCGGGATG
CCAAACGTGGAACTCATTCGTCGAGGAAATCGAGCCGGTCAGAACCGCTGTGCGTGGGGCATTTCTCCATTCGGAGCCGT
CCGGGTCCGCTAAACCGGTTCCGATTACTGCCGTTGCCGCGGTGCAAGAGCCGCGCATCGAAACGAACAGTGCAGAGTTC
AATCGCGTGCTCGGTGGGGGGATCGTCAAAGGGTCGCTCGTCTTAATCGGCGGCGACCCCGGCATCGGCAAGTCGACGCT
GTTGCTGCAGGCCTCAGCGCAACTGGCGGCTAGGCATACAGTGTTGTATGTATCGGGCGAGGAATCCGTCAAACAGGTAA
AACTGCGCGCTGGGCGCCTTCGCGCCGAGTCCGATCATCTGTATGTGTTAGCGGAGGCGAATTTGGAATATATTGTATCA
GCGATTGAAACGATTCAGCCCTCCTGTGTCGTGATTGATTCAATCCAGACGGTGTACCGGACGGATATTACGTCAGCGCC
GGGCAGTGTCGCCCAAGTGCGCGAGTGCACCGCTGAGTTGATGAAAATCGCCAAAACAAAAGGCGTCGCCATTTTTATCG
TCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGCATTCTCGAGCATATGGTCGATACCGTCCTTTATTTTGAG
GGGGAGCGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGTATTTTTGA
AATGCGCGACATCGGGCTGCGGGAAGTGGAAAATCCGTCCGAAGTGTTTTTGGAAGAGCGATCGCACGGGGCGGCGGGTT
CGACGGTGGTGGCGGCCATGGAAGGGACGCGCCCGGTTCTGGTGGAAATTCAAGCGCTCGTTTCGCCGACAAGCTTCGGC
AACCCGAGGCGGATGGCGACCGGCCTCGATCATAACCGCGTGTCGTTGCTTATGGCCGTATTGGAAAAGCGGGTCGGGCT
GCTGTTGCAAAACCAAGATGCCTATTTGAAGGTGGCGGGCGGAGTGAAGCTTGATGAGCCGGCCATTGACCTAGCCATCG
CCGTCAGCATCGCCTCAAGCTTTCGCGATCGGCCGACCAATCCGACCGACGTCATTATCGGTGAAGTCGGGTTGACCGGG
GAAGTGCGGCGTGTCTCCCGCATTGAACAGCGTGTGCAAGAGGCTGTCAAACTAGGTTTTTCCCGTGTCATTGTACCGAA
AAACAATTTGGCAGGTTGGCAGCCGCCGGCAAGCGTTCAGGTGATCGGCGTTTCCCATGTGGCTGAGGCGCTCGAGCATA
CGATGATTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-221)

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150N5A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.179

100

0.743
  radA Streptococcus mitis NCTC 12261

64.68

99.342

0.643
  radA Streptococcus mitis SK321

64.68

99.342

0.643
  radA Streptococcus pneumoniae Rx1

64.459

99.342

0.64
  radA Streptococcus pneumoniae D39

64.459

99.342

0.64
  radA Streptococcus pneumoniae R6

64.459

99.342

0.64
  radA Streptococcus pneumoniae TIGR4

64.459

99.342

0.64