Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V4941_RS08220 Genome accession   NZ_CP144736
Coordinates   1885025..1886533 (+) Length   502 a.a.
NCBI ID   WP_041806998.1    Uniprot ID   P08623
Organism   Azotobacter vinelandii DJ     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1880025..1891533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4941_RS08190 (V4941_08185) - 1880509..1881318 (-) 810 WP_012699931.1 ATP-binding cassette domain-containing protein -
  V4941_RS08195 (V4941_08190) - 1881543..1882517 (+) 975 WP_041807000.1 KpsF/GutQ family sugar-phosphate isomerase -
  V4941_RS08200 (V4941_08195) - 1882519..1883043 (+) 525 WP_012699929.1 HAD family hydrolase -
  V4941_RS08205 (V4941_08200) lptC 1883052..1883627 (+) 576 WP_041807512.1 LPS export ABC transporter periplasmic protein LptC -
  V4941_RS08210 (V4941_08205) lptA 1883614..1884168 (+) 555 WP_012699927.1 lipopolysaccharide transport periplasmic protein LptA -
  V4941_RS08215 (V4941_08210) lptB 1884168..1884893 (+) 726 WP_012699926.1 LPS export ABC transporter ATP-binding protein -
  V4941_RS08220 (V4941_08215) rpoN/rpoN1 1885025..1886533 (+) 1509 WP_041806998.1 RNA polymerase factor sigma-54 Machinery gene
  V4941_RS08225 (V4941_08220) raiA 1886653..1886961 (+) 309 WP_012699924.1 ribosome-associated translation inhibitor RaiA -
  V4941_RS08230 (V4941_08225) ptsN 1886974..1887438 (+) 465 WP_012699923.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V4941_RS08235 (V4941_08230) rapZ 1887534..1888391 (+) 858 WP_012699922.1 RNase adapter RapZ -
  V4941_RS08240 (V4941_08235) - 1888413..1888685 (+) 273 WP_012699921.1 HPr family phosphocarrier protein -
  V4941_RS08245 (V4941_08240) pmbA 1888745..1890091 (-) 1347 WP_012699920.1 metalloprotease PmbA -
  V4941_RS08250 (V4941_08245) yjgA 1890169..1890687 (+) 519 WP_012699919.1 ribosome biogenesis factor YjgA -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 56917.07 Da        Isoelectric Point: 4.4589

>NTDB_id=847615 V4941_RS08220 WP_041806998.1 1885025..1886533(+) (rpoN/rpoN1) [Azotobacter vinelandii DJ]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPMLERQEDAEDYDSPDMLGEHGDQSTLDTTPGSYQE
GYESGAASEDGGTLEEGDWHERIPSELPVDTAWEDIYQTSASNLPSTDEDEWDFTTRTSTGESLQSHLLWQLNLTPMSDT
DRLIAVTLIDSINSDGYLEAALEEILASLDPELGVELDEVEMVLRRIQQFEPAGIAARDLSESLLLQLRQLPPDTPWLEE
AKRLAKDYLDLLGNRDFTQLMRRMKLKEEELRPVIELIQSLNPRPGAQIESSEPEYVVPDVIVRKHNDRWLVELNQEAVP
RLRINPHYAGFIRRADASADNTFMRNQLQEARWFIKSLQSRNETLMKVSTQIVEHQRGFLDYGEEAMKPLVLHDIAEAVG
MHESTISRVTTQKYMHTPRGIYELKYFFSSHVSTAEGGECSSTAIRAIIKKLIAAENPKKPLSDSKIAGLLEEQGIQVAR
RTVAKYRESLSIAPSSERKRLM

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=847615 V4941_RS08220 WP_041806998.1 1885025..1886533(+) (rpoN/rpoN1) [Azotobacter vinelandii DJ]
ATGAAACCATCGCTAGTCCTTAAGATGGGGCAGCAGCTGACGATGACCCCGCAGCTGCAACAAGCCATCCGTCTGCTTCA
ACTGTCGACCCTGGATCTGCAGCAGGAAATCCAGGAGGCCCTCGACTCCAATCCCATGCTGGAACGTCAGGAGGACGCCG
AGGACTACGACAGCCCGGATATGCTGGGCGAGCATGGAGACCAGTCGACGCTCGACACCACGCCCGGCTCTTACCAGGAA
GGCTACGAGAGCGGGGCGGCCAGCGAGGATGGCGGTACCCTCGAAGAGGGCGACTGGCACGAGCGGATTCCCAGCGAGCT
GCCGGTGGATACCGCCTGGGAAGACATCTACCAGACCAGTGCCAGCAACCTGCCGAGCACCGATGAAGACGAGTGGGACT
TCACCACCCGCACCTCCACGGGCGAGAGCCTGCAGAGCCATCTGCTCTGGCAGTTGAACCTGACCCCGATGTCGGATACC
GATCGCCTGATCGCCGTCACTCTGATCGACAGCATCAACAGCGACGGCTATCTGGAGGCCGCCCTGGAGGAAATCCTCGC
CTCTCTGGACCCGGAACTGGGAGTCGAACTCGACGAAGTGGAAATGGTGCTGCGCCGCATCCAGCAATTCGAACCGGCCG
GGATCGCTGCCCGCGACCTCAGCGAATCGCTGCTGCTGCAACTGCGCCAGCTACCGCCCGATACCCCCTGGCTGGAAGAG
GCGAAACGACTGGCCAAGGACTATCTCGACCTGCTGGGTAACCGCGACTTCACCCAGTTGATGCGACGCATGAAACTCAA
GGAAGAAGAATTGCGTCCGGTGATCGAGCTGATCCAGAGCCTCAACCCTCGTCCCGGGGCCCAGATCGAGAGCAGCGAGC
CCGAATATGTCGTGCCTGACGTCATCGTGCGCAAGCACAACGACCGCTGGCTGGTGGAGCTCAATCAGGAGGCGGTGCCG
CGCCTGCGCATCAACCCGCATTACGCTGGCTTCATCAGACGCGCCGACGCCAGCGCCGACAACACCTTCATGCGCAACCA
ACTGCAGGAGGCGCGCTGGTTCATCAAGAGCCTGCAAAGTCGCAACGAAACCCTGATGAAGGTTTCGACCCAAATCGTCG
AGCACCAGCGCGGCTTTCTCGACTACGGCGAAGAGGCCATGAAACCGCTGGTGCTGCACGATATCGCCGAGGCTGTCGGC
ATGCACGAATCGACCATCTCCAGGGTCACCACCCAGAAATACATGCACACTCCACGCGGTATTTACGAGCTGAAGTACTT
CTTTTCCAGTCACGTCAGTACCGCCGAAGGCGGTGAGTGCTCGTCCACGGCCATCCGCGCCATCATCAAGAAATTGATTG
CGGCGGAAAATCCGAAAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAAGAACAAGGCATACAGGTGGCTCGC
CGTACAGTTGCCAAATACCGGGAATCGCTCAGTATTGCGCCTTCCAGCGAACGCAAGCGGCTTATGTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(342-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P08623

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.328

100

0.454