Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V4939_RS05010 Genome accession   NZ_CP144733
Coordinates   1068836..1070344 (-) Length   502 a.a.
NCBI ID   WP_041806998.1    Uniprot ID   P08623
Organism   Azotobacter vinelandii DJ     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1063836..1075344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4939_RS04980 (V4939_04980) yjgA 1064682..1065200 (-) 519 WP_012699919.1 ribosome biogenesis factor YjgA -
  V4939_RS04985 (V4939_04985) pmbA 1065278..1066624 (+) 1347 WP_012699920.1 metalloprotease PmbA -
  V4939_RS04990 (V4939_04990) - 1066684..1066956 (-) 273 WP_012699921.1 HPr family phosphocarrier protein -
  V4939_RS04995 (V4939_04995) rapZ 1066978..1067835 (-) 858 WP_012699922.1 RNase adapter RapZ -
  V4939_RS05000 (V4939_05000) ptsN 1067931..1068395 (-) 465 WP_012699923.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V4939_RS05005 (V4939_05005) raiA 1068408..1068716 (-) 309 WP_012699924.1 ribosome-associated translation inhibitor RaiA -
  V4939_RS05010 (V4939_05010) rpoN/rpoN1 1068836..1070344 (-) 1509 WP_041806998.1 RNA polymerase factor sigma-54 Machinery gene
  V4939_RS05015 (V4939_05015) lptB 1070476..1071201 (-) 726 WP_012699926.1 LPS export ABC transporter ATP-binding protein -
  V4939_RS05020 (V4939_05020) lptA 1071201..1071755 (-) 555 WP_012699927.1 lipopolysaccharide transport periplasmic protein LptA -
  V4939_RS05025 (V4939_05025) lptC 1071742..1072317 (-) 576 WP_041807512.1 LPS export ABC transporter periplasmic protein LptC -
  V4939_RS05030 (V4939_05030) - 1072326..1072850 (-) 525 WP_012699929.1 HAD family hydrolase -
  V4939_RS05035 (V4939_05035) - 1072852..1073826 (-) 975 WP_041807000.1 KpsF/GutQ family sugar-phosphate isomerase -
  V4939_RS05040 (V4939_05040) - 1074051..1074860 (+) 810 WP_012699931.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 56917.07 Da        Isoelectric Point: 4.4589

>NTDB_id=847523 V4939_RS05010 WP_041806998.1 1068836..1070344(-) (rpoN/rpoN1) [Azotobacter vinelandii DJ]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPMLERQEDAEDYDSPDMLGEHGDQSTLDTTPGSYQE
GYESGAASEDGGTLEEGDWHERIPSELPVDTAWEDIYQTSASNLPSTDEDEWDFTTRTSTGESLQSHLLWQLNLTPMSDT
DRLIAVTLIDSINSDGYLEAALEEILASLDPELGVELDEVEMVLRRIQQFEPAGIAARDLSESLLLQLRQLPPDTPWLEE
AKRLAKDYLDLLGNRDFTQLMRRMKLKEEELRPVIELIQSLNPRPGAQIESSEPEYVVPDVIVRKHNDRWLVELNQEAVP
RLRINPHYAGFIRRADASADNTFMRNQLQEARWFIKSLQSRNETLMKVSTQIVEHQRGFLDYGEEAMKPLVLHDIAEAVG
MHESTISRVTTQKYMHTPRGIYELKYFFSSHVSTAEGGECSSTAIRAIIKKLIAAENPKKPLSDSKIAGLLEEQGIQVAR
RTVAKYRESLSIAPSSERKRLM

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=847523 V4939_RS05010 WP_041806998.1 1068836..1070344(-) (rpoN/rpoN1) [Azotobacter vinelandii DJ]
ATGAAACCATCGCTAGTCCTTAAGATGGGGCAGCAGCTGACGATGACCCCGCAGCTGCAACAAGCCATCCGTCTGCTTCA
ACTGTCGACCCTGGATCTGCAGCAGGAAATCCAGGAGGCCCTCGACTCCAATCCCATGCTGGAACGTCAGGAGGACGCCG
AGGACTACGACAGCCCGGATATGCTGGGCGAGCATGGAGACCAGTCGACGCTCGACACCACGCCCGGCTCTTACCAGGAA
GGCTACGAGAGCGGGGCGGCCAGCGAGGATGGCGGTACCCTCGAAGAGGGCGACTGGCACGAGCGGATTCCCAGCGAGCT
GCCGGTGGATACCGCCTGGGAAGACATCTACCAGACCAGTGCCAGCAACCTGCCGAGCACCGATGAAGACGAGTGGGACT
TCACCACCCGCACCTCCACGGGCGAGAGCCTGCAGAGCCATCTGCTCTGGCAGTTGAACCTGACCCCGATGTCGGATACC
GATCGCCTGATCGCCGTCACTCTGATCGACAGCATCAACAGCGACGGCTATCTGGAGGCCGCCCTGGAGGAAATCCTCGC
CTCTCTGGACCCGGAACTGGGAGTCGAACTCGACGAAGTGGAAATGGTGCTGCGCCGCATCCAGCAATTCGAACCGGCCG
GGATCGCTGCCCGCGACCTCAGCGAATCGCTGCTGCTGCAACTGCGCCAGCTACCGCCCGATACCCCCTGGCTGGAAGAG
GCGAAACGACTGGCCAAGGACTATCTCGACCTGCTGGGTAACCGCGACTTCACCCAGTTGATGCGACGCATGAAACTCAA
GGAAGAAGAATTGCGTCCGGTGATCGAGCTGATCCAGAGCCTCAACCCTCGTCCCGGGGCCCAGATCGAGAGCAGCGAGC
CCGAATATGTCGTGCCTGACGTCATCGTGCGCAAGCACAACGACCGCTGGCTGGTGGAGCTCAATCAGGAGGCGGTGCCG
CGCCTGCGCATCAACCCGCATTACGCTGGCTTCATCAGACGCGCCGACGCCAGCGCCGACAACACCTTCATGCGCAACCA
ACTGCAGGAGGCGCGCTGGTTCATCAAGAGCCTGCAAAGTCGCAACGAAACCCTGATGAAGGTTTCGACCCAAATCGTCG
AGCACCAGCGCGGCTTTCTCGACTACGGCGAAGAGGCCATGAAACCGCTGGTGCTGCACGATATCGCCGAGGCTGTCGGC
ATGCACGAATCGACCATCTCCAGGGTCACCACCCAGAAATACATGCACACTCCACGCGGTATTTACGAGCTGAAGTACTT
CTTTTCCAGTCACGTCAGTACCGCCGAAGGCGGTGAGTGCTCGTCCACGGCCATCCGCGCCATCATCAAGAAATTGATTG
CGGCGGAAAATCCGAAAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAAGAACAAGGCATACAGGTGGCTCGC
CGTACAGTTGCCAAATACCGGGAATCGCTCAGTATTGCGCCTTCCAGCGAACGCAAGCGGCTTATGTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(342-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P08623

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.328

100

0.454