Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QRA13_RS10535 Genome accession   NZ_CP128184
Coordinates   2208023..2208460 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain YJ0-1 isolate soil     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2203023..2213460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRA13_RS10520 (QRA13_10520) - 2205881..2206243 (-) 363 WP_007409352.1 helix-turn-helix domain-containing protein -
  QRA13_RS10525 (QRA13_10525) hxlA 2206475..2207110 (+) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  QRA13_RS10530 (QRA13_10530) hxlB 2207107..2207664 (+) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  QRA13_RS10535 (QRA13_10535) nucA/comI 2208023..2208460 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  QRA13_RS10540 (QRA13_10540) nin/comJ 2208481..2208879 (+) 399 WP_007609299.1 competence protein ComJ Regulator
  QRA13_RS10545 (QRA13_10545) - 2208920..2210356 (-) 1437 WP_076425807.1 family 1 glycosylhydrolase -
  QRA13_RS10550 (QRA13_10550) - 2210460..2210783 (-) 324 WP_007409357.1 YckD family protein -
  QRA13_RS10555 (QRA13_10555) - 2210856..2211290 (-) 435 WP_094031491.1 RDD family protein -
  QRA13_RS10560 (QRA13_10560) - 2211305..2212441 (-) 1137 WP_094031490.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=846504 QRA13_RS10535 WP_003156588.1 2208023..2208460(+) (nucA/comI) [Bacillus velezensis strain YJ0-1 isolate soil]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=846504 QRA13_RS10535 WP_003156588.1 2208023..2208460(+) (nucA/comI) [Bacillus velezensis strain YJ0-1 isolate soil]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641