Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QRE62_RS25060 Genome accession   NZ_CP128149
Coordinates   4166702..4167745 (-) Length   347 a.a.
NCBI ID   WP_002014996.1    Uniprot ID   R8I3I9
Organism   Bacillus mycoides strain SIN3.2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4161702..4172745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRE62_RS25015 (QRE62_25010) - 4162099..4162299 (-) 201 WP_002190662.1 YqzE family protein -
  QRE62_RS25020 (QRE62_25015) - 4162338..4162835 (-) 498 WP_002190663.1 shikimate kinase -
  QRE62_RS25025 (QRE62_25020) - 4162987..4163637 (-) 651 WP_286126198.1 2OG-Fe(II) oxygenase -
  QRE62_RS25030 (QRE62_25025) comGG 4163811..4164182 (-) 372 WP_286126199.1 competence type IV pilus minor pilin ComGG -
  QRE62_RS25035 (QRE62_25030) comGF 4164179..4164642 (-) 464 Protein_4162 competence type IV pilus minor pilin ComGF -
  QRE62_RS25040 (QRE62_25035) - 4164626..4164922 (-) 297 WP_002190667.1 hypothetical protein -
  QRE62_RS25045 (QRE62_25040) comGD 4164915..4165370 (-) 456 WP_002088622.1 competence type IV pilus minor pilin ComGD -
  QRE62_RS25050 (QRE62_25045) comGC 4165367..4165666 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  QRE62_RS25055 (QRE62_25050) comGB 4165678..4166715 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  QRE62_RS25060 (QRE62_25055) comGA 4166702..4167745 (-) 1044 WP_002014996.1 competence type IV pilus ATPase ComGA Machinery gene
  QRE62_RS25065 (QRE62_25060) - 4167951..4168646 (+) 696 WP_002014995.1 helix-turn-helix domain-containing protein -
  QRE62_RS25070 (QRE62_25065) - 4168926..4169168 (+) 243 WP_002190671.1 DUF2626 domain-containing protein -
  QRE62_RS25075 (QRE62_25070) - 4169276..4170670 (+) 1395 WP_002138338.1 L-cystine transporter -
  QRE62_RS25080 (QRE62_25075) - 4170751..4171143 (-) 393 WP_002165071.1 hypothetical protein -
  QRE62_RS25085 (QRE62_25080) - 4171231..4171446 (-) 216 WP_002034026.1 DUF3912 family protein -
  QRE62_RS25090 (QRE62_25085) - 4171699..4172010 (+) 312 WP_002165070.1 hypothetical protein -
  QRE62_RS25095 (QRE62_25090) - 4172046..4172534 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39264.73 Da        Isoelectric Point: 9.3368

>NTDB_id=846256 QRE62_RS25060 WP_002014996.1 4166702..4167745(-) (comGA) [Bacillus mycoides strain SIN3.2]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=846256 QRE62_RS25060 WP_002014996.1 4166702..4167745(-) (comGA) [Bacillus mycoides strain SIN3.2]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGACTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8I3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363