Detailed information NTG0084617

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ST1232TP_RS07900
• Genome accession: NZ_AP024311
• Coordinates: 1641612..1642109 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain THI2018-120
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1636612..1647109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST1232TP_RS07875 (THI2018120TP_15210)	gcvPB	1636774..1638246 (-)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ST1232TP_RS07880 (THI2018120TP_15220)	gcvPA	1638239..1639585 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ST1232TP_RS07885 (THI2018120TP_15230)	gcvT	1639605..1640696 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ST1232TP_RS07890 (THI2018120TP_15240)	-	1640855..1641379 (-)	525	WP_001015120.1	shikimate kinase	-
ST1232TP_RS07895	-	1641369..1641515 (-)	147	WP_001789879.1	hypothetical protein	-
ST1232TP_RS07900 (THI2018120TP_15250)	comGF	1641612..1642109 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ST1232TP_RS07905 (THI2018120TP_15260)	comGE	1642027..1642326 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ST1232TP_RS07910 (THI2018120TP_15270)	comGD	1642313..1642759 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ST1232TP_RS07915 (THI2018120TP_15280)	comGC	1642737..1643048 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ST1232TP_RS07920 (THI2018120TP_15290)	comGB	1643062..1644132 (-)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ST1232TP_RS07925 (THI2018120TP_15300)	comGA	1644104..1645078 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ST1232TP_RS07930 (THI2018120TP_15310)	-	1645130..1645753 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ST1232TP_RS07935 (THI2018120TP_15320)	-	1645750..1646079 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ST1232TP_RS07940 (THI2018120TP_15330)	-	1646079..1647065 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=84617 ST1232TP_RS07900 WP_001825340.1 1641612..1642109(-) (comGF) [Staphylococcus aureus strain THI2018-120]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=84617 ST1232TP_RS07900 WP_001825340.1 1641612..1642109(-) (comGF) [Staphylococcus aureus strain THI2018-120]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97