Detailed information NTG0845781

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QRY07_RS06315
• Genome accession: NZ_CP128107
• Coordinates: 1137630..1141355 (+)
• Length: 1241 a.a.
• NCBI ID: WP_215597297.1
• Uniprot ID: -
• Organism: Bacillus cereus strain D5_B_69
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1132630..1146355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRY07_RS06300 (QRY07_06300)	-	1132788..1133381 (+)	594	WP_002201415.1	TVP38/TMEM64 family protein	-
QRY07_RS06305 (QRY07_06305)	lepB	1133437..1134000 (+)	564	WP_002158924.1	signal peptidase I	-
QRY07_RS06310 (QRY07_06310)	addB	1134118..1137633 (+)	3516	WP_002201414.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QRY07_RS06315 (QRY07_06315)	addA	1137630..1141355 (+)	3726	WP_215597297.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QRY07_RS06320 (QRY07_06320)	-	1141379..1141657 (+)	279	WP_002201412.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
QRY07_RS06325 (QRY07_06325)	-	1141874..1142581 (+)	708	WP_002201411.1	DNA alkylation repair protein	-
QRY07_RS06330 (QRY07_06330)	-	1142851..1143336 (+)	486	WP_002201410.1	hypothetical protein	-
QRY07_RS06335 (QRY07_06335)	gerPF	1143422..1143637 (-)	216	WP_001141570.1	spore germination protein GerPF	-
QRY07_RS06340 (QRY07_06340)	-	1143680..1144066 (-)	387	WP_002140807.1	spore germination protein GerPE	-
QRY07_RS06345 (QRY07_06345)	gerPD	1144081..1144275 (-)	195	WP_001052805.1	spore germination protein GerPD	-
QRY07_RS06350 (QRY07_06350)	-	1144282..1144896 (-)	615	WP_002158914.1	spore germination protein GerPC	-
QRY07_RS06355 (QRY07_06355)	gerPB	1144961..1145167 (-)	207	WP_002201409.1	spore germination protein GerPB	-
QRY07_RS06360 (QRY07_06360)	gerPA	1145182..1145403 (-)	222	WP_002201408.1	spore germination protein GerPA	-
QRY07_RS06365 (QRY07_06365)	-	1145500..1145679 (-)	180	WP_002087032.1	aspartyl-phosphate phosphatase Spo0E family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142606.76 Da
• Isoelectric Point: 4.8528

>NTDB_id=845781 QRY07_RS06315 WP_215597297.1 1137630..1141355(+) (addA) [Bacillus cereus strain D5_B_69]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEEHI
RKATELAMLPDGPAPRAETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYKLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYGYSASWK
VEVVDGKTLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKSDESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPTL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=845781 QRY07_RS06315 WP_215597297.1 1137630..1141355(+) (addA) [Bacillus cereus strain D5_B_69]
ATGATAGAAAATTGGCCTGAGAAACCAGAAGGTAGTCAATGGACAGATGATCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAAATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAACAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACCATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGATGTTGAAGGAA
AGACGATTGAAGATTTGGTTTACGCTTCTTACCTATTAGAAGATGTAAAGTTTCAGCTTGAAACAGCGGAAGAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGTGCCGAAACTTTACAAGCGGATTTAGCTTTACT
TGGAACGTTATCATCAGCAGCTCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGAGCAAAGTGAAGATGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCTGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACTGAGGAAGAAGTGCAAGAAGGTGAAGAAGGTACAGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCGGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAAATTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAATCTCTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
ACGCGTATTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGATTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGACATGGGAACGGCGAGAGCTCTTGGAGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAGGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCATGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGGAATATTCCAGGCGAAATTTATGGGTATAGTGCGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
CGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGGAATATGGGTATGAGG
ACGCGACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTTCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAACGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGTAAGAGTGACGAATCGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAGACAGATACGATTGAAGGGAAGTTCCCAGGTGGATTTGATCAAGCGAAACCAACTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.729	100	0.54