Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   JQN54_RS07140 Genome accession   NZ_AP024305
Coordinates   1344199..1344669 (+) Length   156 a.a.
NCBI ID   WP_203970909.1    Uniprot ID   -
Organism   Thermus thermophilus strain HB5008     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1339199..1349669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQN54_RS07120 (TthHB5008_14280) ilvC 1339699..1340712 (-) 1014 WP_011173279.1 ketol-acid reductoisomerase -
  JQN54_RS07125 (TthHB5008_14290) ilvN 1340709..1341221 (-) 513 WP_096412614.1 acetolactate synthase small subunit -
  JQN54_RS07130 (TthHB5008_14300) ilvB 1341218..1342906 (-) 1689 WP_024119431.1 biosynthetic-type acetolactate synthase large subunit -
  JQN54_RS07135 (TthHB5008_14310) - 1343061..1344041 (-) 981 WP_024119432.1 YpdA family putative bacillithiol disulfide reductase -
  JQN54_RS07140 (TthHB5008_14320) pilA/pilA1 1344199..1344669 (+) 471 WP_203970909.1 GspH/FimT family protein Machinery gene
  JQN54_RS07145 (TthHB5008_14330) - 1344724..1345191 (+) 468 WP_011228523.1 GspH/FimT family protein -
  JQN54_RS07150 (TthHB5008_14340) - 1345217..1345549 (+) 333 WP_224065211.1 prepilin -
  JQN54_RS07155 (TthHB5008_14350) - 1345546..1346256 (+) 711 WP_011228525.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JQN54_RS07160 (TthHB5008_14360) - 1346270..1347769 (+) 1500 WP_203970910.1 PilX N-terminal domain-containing pilus assembly protein -
  JQN54_RS07165 (TthHB5008_14370) - 1348033..1348407 (+) 375 WP_203970911.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16903.71 Da        Isoelectric Point: 11.7108

>NTDB_id=84570 JQN54_RS07140 WP_203970909.1 1344199..1344669(+) (pilA/pilA1) [Thermus thermophilus strain HB5008]
MTRRGLTLLELLLVLGILGVLLGLALPLLSPNRLALDQAARSLATQVTRARLEAIRRNAFVGLQVFTEGAGGYLLFVDQN
ANRRYDPGEEFGATRFGQGNWARVRLDPEKSALGNMPLLFDPRGIPAKPITATLVLTSGGATRKVVISQQGRARLE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=84570 JQN54_RS07140 WP_203970909.1 1344199..1344669(+) (pilA/pilA1) [Thermus thermophilus strain HB5008]
ATGACTCGGCGGGGCCTTACCCTTTTGGAGCTCCTGCTGGTTTTGGGCATCCTGGGGGTCCTCCTAGGGCTGGCCCTTCC
ACTTCTTTCCCCCAACCGCCTCGCCCTGGACCAGGCCGCCCGCTCCCTCGCCACCCAGGTGACCCGGGCAAGGCTCGAGG
CCATCCGCCGGAACGCCTTCGTGGGCCTTCAGGTGTTCACCGAAGGAGCGGGGGGTTACCTGCTCTTCGTGGACCAAAAC
GCCAACCGCCGCTACGACCCCGGGGAGGAGTTCGGCGCGACCCGCTTCGGCCAAGGGAACTGGGCCCGGGTGCGCCTGGA
CCCGGAGAAGAGCGCCCTTGGCAACATGCCCCTTCTCTTTGACCCCCGGGGCATCCCGGCCAAGCCCATCACCGCCACGC
TCGTCCTCACCTCAGGGGGCGCCACCCGCAAGGTGGTCATCTCCCAGCAGGGCCGGGCCCGCCTGGAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)

Type II transport protein GspH (GspH)

(39-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

99.359

100

0.994

Multiple sequence alignment