Detailed information NTG0845633

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QPE33_RS15270
• Genome accession: NZ_CP128003
• Coordinates: 3068408..3069208 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 2018S09-085
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3063408..3074208

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QPE33_RS15235 (QPE33_15200)	gspH	3063564..3064079 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
QPE33_RS15240 (QPE33_15205)	gspI	3064076..3064441 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
QPE33_RS15245 (QPE33_15210)	gspJ	3064438..3065034 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
QPE33_RS15250 (QPE33_15215)	gspK	3065031..3066017 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
QPE33_RS15255 (QPE33_15220)	gspL	3066004..3067197 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
QPE33_RS15260 (QPE33_15225)	-	3067190..3067675 (+)	486	WP_004151943.1	type II secretion system protein M	-
QPE33_RS15265 (QPE33_15230)	-	3067665..3068411 (+)	747	WP_004145897.1	type II secretion system protein N	-
QPE33_RS15270 (QPE33_15235)	pilD	3068408..3069208 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
QPE33_RS15275 (QPE33_15240)	hrpB	3069256..3071685 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
QPE33_RS15280 (QPE33_15245)	thpR	3071758..3072294 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
QPE33_RS15285 (QPE33_15250)	sfsA	3072294..3073010 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
QPE33_RS15290 (QPE33_15255)	dksA	3073173..3073628 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=845633 QPE33_RS15270 WP_002888947.1 3068408..3069208(+) (pilD) [Klebsiella pneumoniae strain 2018S09-085]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=845633 QPE33_RS15270 WP_002888947.1 3068408..3069208(+) (pilD) [Klebsiella pneumoniae strain 2018S09-085]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425