Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VXF94_RS14185 Genome accession   NZ_CP143956
Coordinates   2785329..2786639 (+) Length   436 a.a.
NCBI ID   WP_330937813.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Fad 108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2780329..2791639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXF94_RS14160 (VXF94_14160) - 2781649..2782257 (+) 609 WP_014471058.1 sugar transferase -
  VXF94_RS14165 (VXF94_14165) - 2782254..2782901 (+) 648 WP_013353682.1 acetyltransferase -
  VXF94_RS14170 (VXF94_14170) - 2782906..2784078 (+) 1173 WP_013353681.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  VXF94_RS14175 (VXF94_14175) - 2784057..2785019 (+) 963 WP_013353680.1 polysaccharide pyruvyl transferase family protein -
  VXF94_RS14180 (VXF94_14180) yvfG 2785033..2785251 (-) 219 WP_003151570.1 protein YvfG -
  VXF94_RS14185 (VXF94_14185) rpoN/rpoN1 2785329..2786639 (+) 1311 WP_330937813.1 RNA polymerase factor sigma-54 Machinery gene
  VXF94_RS14190 (VXF94_14190) lutP 2786677..2788352 (-) 1676 Protein_2751 L-lactate permease LutP -
  VXF94_RS14195 (VXF94_14195) - 2788557..2789276 (+) 720 WP_013353677.1 FadR/GntR family transcriptional regulator -
  VXF94_RS14200 (VXF94_14200) - 2789307..2790497 (+) 1191 Protein_2753 PLP-dependent aminotransferase family protein -
  VXF94_RS14205 (VXF94_14205) - 2790679..2791395 (+) 717 WP_013353675.1 (Fe-S)-binding protein -

Sequence

Protein


Download         Length: 436 a.a.        Molecular weight: 49878.02 Da        Isoelectric Point: 9.4753

>NTDB_id=845620 VXF94_RS14185 WP_330937813.1 2785329..2786639(+) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain Fad 108]
MDIKLQQTQILKPQLTQELKQAITLLGYNSAELAGYIDELSLENPLIERKETDTPPLSYHKTNKNRMKEQDVSQQIRLTR
KTLQDSLKQQAIDMKLSDAEKKIFNYLIYSLDSNGYLKEDIQAAADHVSASLEETEAVLAKLQSLEPAGIGARSLQECIL
LQLRRLPERNEQAEWIAGAYFELFARKNWKALTLQTGIPLKDIQDIFDMISALDPRPGLRYAHEEPGFYVEPDIFITVKN
GIIQARLNSRSFPDIQLHAHYRPMLSAAGCRDTKDYLSAKYQEWRWLNRALSQRKQTITRIIGVLTERQASFFLTGKSGM
QPLTLREVAETLELHESTISRAIKGKYVQTPYGLYDMKTFFSAKAESSGNGSASNYAVKAHLEALVRKEDKKKPFSDQKL
ADLLLEQYDIRISRRTVAKYRDQLNIPPSSARKRYS

Nucleotide


Download         Length: 1311 bp        

>NTDB_id=845620 VXF94_RS14185 WP_330937813.1 2785329..2786639(+) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain Fad 108]
ATGGATATCAAACTTCAGCAAACACAAATTTTAAAGCCTCAATTGACTCAAGAGCTTAAACAGGCCATCACTTTACTCGG
ATACAATTCCGCGGAACTGGCCGGGTATATTGATGAGCTCTCTCTGGAAAACCCTCTGATTGAACGAAAGGAAACAGACA
CGCCGCCACTATCTTACCATAAAACAAATAAAAACAGGATGAAAGAACAGGACGTAAGCCAGCAGATCAGGCTTACGCGC
AAAACGCTGCAGGATTCGCTCAAACAGCAGGCCATAGACATGAAGCTGTCCGATGCTGAAAAGAAAATTTTTAATTATCT
GATATATTCCTTGGATTCTAACGGATATTTAAAAGAGGACATACAAGCTGCCGCCGACCATGTATCCGCTTCACTAGAGG
AGACGGAAGCCGTTCTGGCAAAGCTTCAATCGCTTGAGCCCGCGGGAATCGGCGCCAGGTCTTTGCAGGAATGCATCCTT
CTTCAGCTCCGCCGTCTGCCCGAGCGGAACGAACAGGCGGAATGGATTGCCGGCGCCTATTTTGAGCTTTTTGCAAGAAA
GAACTGGAAAGCGCTTACATTGCAAACGGGCATTCCGCTCAAAGACATTCAGGACATCTTTGACATGATCAGCGCGCTTG
ACCCGAGGCCGGGGCTCCGTTATGCGCATGAGGAACCGGGTTTTTACGTTGAACCGGATATTTTCATCACGGTGAAAAAC
GGCATCATTCAAGCGCGGCTCAACAGCCGTTCGTTCCCGGATATCCAGCTTCATGCCCATTATCGGCCGATGCTGTCAGC
CGCCGGATGCCGTGACACGAAAGATTATCTGTCGGCAAAATATCAGGAATGGCGCTGGCTGAACCGGGCTTTATCGCAAA
GAAAACAAACGATCACGAGAATTATCGGCGTCCTGACTGAACGGCAGGCATCCTTTTTTCTCACAGGAAAAAGCGGCATG
CAGCCGCTGACCTTAAGAGAGGTGGCGGAAACGCTGGAGCTCCATGAATCAACGATCAGCCGGGCCATCAAAGGAAAGTA
CGTGCAGACGCCGTACGGACTGTACGATATGAAAACATTTTTTTCGGCGAAAGCTGAATCATCGGGAAACGGCAGCGCCT
CAAACTATGCCGTAAAAGCACACCTGGAAGCATTGGTCCGTAAAGAAGACAAAAAGAAACCTTTTTCAGACCAAAAGCTT
GCCGACCTGCTGCTTGAACAATATGACATCCGAATTTCAAGACGCACCGTCGCCAAATACCGTGACCAATTAAATATCCC
GCCGTCATCGGCGAGAAAACGATACAGCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(77-261)

Sigma-54, DNA binding domain (Sigma54_DBD)

(275-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

32.803

100

0.378