Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QSG87_RS14660 Genome accession   NZ_CP127923
Coordinates   3011055..3011840 (-) Length   261 a.a.
NCBI ID   WP_394357280.1    Uniprot ID   -
Organism   Acinetobacter chinensis strain SAAc573     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3006055..3016840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG87_RS14640 (QSG87_14640) - 3007102..3007800 (-) 699 WP_087551345.1 tetratricopeptide repeat protein -
  QSG87_RS14645 (QSG87_14645) hisS 3007830..3009125 (-) 1296 WP_317023478.1 histidine--tRNA ligase -
  QSG87_RS14650 (QSG87_14650) ispG 3009129..3010250 (-) 1122 WP_317023479.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QSG87_RS14655 (QSG87_14655) - 3010276..3011064 (-) 789 WP_087513065.1 DUF4115 domain-containing protein -
  QSG87_RS14660 (QSG87_14660) pilF 3011055..3011840 (-) 786 WP_394357280.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QSG87_RS14665 (QSG87_14665) rlmN 3011878..3013113 (-) 1236 WP_087551350.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QSG87_RS14670 (QSG87_14670) ndk 3013235..3013666 (-) 432 WP_317023481.1 nucleoside-diphosphate kinase -
  QSG87_RS14675 (QSG87_14675) iscX 3013788..3013988 (-) 201 WP_087513069.1 Fe-S cluster assembly protein IscX -
  QSG87_RS14680 (QSG87_14680) - 3014078..3014629 (-) 552 WP_317023482.1 phosphatase PAP2 family protein -
  QSG87_RS14685 (QSG87_14685) - 3014662..3015948 (-) 1287 WP_317023483.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 261 a.a.        Molecular weight: 29077.87 Da        Isoelectric Point: 8.8058

>NTDB_id=845252 QSG87_RS14660 WP_394357280.1 3011055..3011840(-) (pilF) [Acinetobacter chinensis strain SAAc573]
MAFITTVCISTVLISGCQTPATIGQKDPEKAVKVRSQLAAEYIRSGDLDAAKRALDQALESDPRDANANMMMGILLQQEG
SKISMEKADSYFRRSISSDSKNAQARNNYGTYLYQVERYNEAVEQLSIAGATLGYDQRSRALENLGRVYLKLGDQASAEK
AFRQALQANRNSFVSMLELSEILYLNQQAESAGKLYEQFVRAVGQKNQGARALWIGIRIARANGDNLGMQVLVNQLRALF
PDSQEYQSYLKLQHSTEAVWK

Nucleotide


Download         Length: 786 bp        

>NTDB_id=845252 QSG87_RS14660 WP_394357280.1 3011055..3011840(-) (pilF) [Acinetobacter chinensis strain SAAc573]
ATTGCATTTATAACAACAGTCTGTATATCAACAGTGTTGATTTCAGGCTGTCAGACTCCCGCTACAATTGGACAGAAAGA
TCCTGAAAAAGCAGTCAAAGTCAGATCTCAGCTGGCGGCTGAATACATTCGCAGTGGTGATCTTGATGCGGCGAAACGTG
CACTGGATCAGGCACTTGAGTCAGACCCACGTGATGCGAATGCCAATATGATGATGGGTATCCTGTTACAGCAGGAAGGC
AGCAAAATCAGCATGGAAAAAGCTGACAGCTATTTCCGCCGTTCCATCTCATCAGATTCCAAAAATGCGCAGGCGCGTAA
CAACTATGGTACGTACTTATATCAGGTTGAGCGTTATAATGAGGCGGTTGAACAACTGAGTATTGCGGGAGCAACGCTTG
GATATGATCAGCGTTCAAGAGCACTTGAAAATCTTGGACGGGTATATCTGAAGCTGGGAGATCAAGCGAGTGCTGAAAAA
GCATTCAGACAGGCACTCCAGGCAAACCGTAATTCTTTTGTTTCAATGCTGGAATTATCAGAAATTCTTTATCTGAACCA
GCAGGCTGAATCCGCAGGGAAGCTGTATGAACAGTTTGTCCGTGCAGTCGGTCAGAAAAATCAGGGAGCGCGTGCATTAT
GGATCGGTATCCGCATCGCACGGGCCAATGGCGATAATCTGGGAATGCAGGTGCTGGTCAATCAGTTACGGGCTCTGTTT
CCAGACAGCCAGGAATACCAAAGTTATTTGAAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(103-170)

Tetratricopeptide repeat (TPR_16)

(38-73)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76

95.785

0.728
  pilF Acinetobacter baylyi ADP1

68.775

96.935

0.667