Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QSG86_RS02045 Genome accession   NZ_CP127915
Coordinates   216621..217370 (-) Length   249 a.a.
NCBI ID   WP_317029985.1    Uniprot ID   -
Organism   Acinetobacter sp. SAAs474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 211621..222370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG86_RS02025 (QSG86_02020) - 212672..213376 (-) 705 WP_317029981.1 YfgM family protein -
  QSG86_RS02030 (QSG86_02025) hisS 213407..214702 (-) 1296 WP_317029982.1 histidine--tRNA ligase -
  QSG86_RS02035 (QSG86_02030) ispG 214699..215814 (-) 1116 WP_317029983.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QSG86_RS02040 (QSG86_02035) - 215839..216630 (-) 792 WP_317029984.1 helix-turn-helix domain-containing protein -
  QSG86_RS02045 (QSG86_02040) pilF 216621..217370 (-) 750 WP_317029985.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QSG86_RS02050 (QSG86_02045) rlmN 217450..218685 (-) 1236 WP_317029986.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QSG86_RS02055 (QSG86_02050) ndk 218808..219239 (-) 432 WP_317029987.1 nucleoside-diphosphate kinase -
  QSG86_RS02060 (QSG86_02055) iscX 219348..219548 (-) 201 WP_317029988.1 Fe-S cluster assembly protein IscX -
  QSG86_RS02065 (QSG86_02060) - 219865..221109 (-) 1245 WP_317029989.1 glycosyltransferase -
  QSG86_RS02070 (QSG86_02065) - 221496..222302 (-) 807 WP_317029990.1 XAC2610-related protein -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 27706.42 Da        Isoelectric Point: 7.3444

>NTDB_id=845197 QSG86_RS02045 WP_317029985.1 216621..217370(-) (pilF) [Acinetobacter sp. SAAs474]
MLGCQNIPNNALKADPAKSLQAHTQLAAEYIKSADYDAASRELDTALGLNARDAYANMMMGVLLQNEGSPANIAKAERYF
KMAIAVEPKNAQIRNNYGTYLYQTGRYNEALAQLEIAGSTLGYDQRYTALESKGRTYMKLGDLTHAESAFQQALNVNPAS
YIAMIELAEIKYLGNSIPAATQLYHQAVTLIGEKNLDSHALWIGIRIARANGNSLERQVLVNQLRALYPSSLEYQRYLQL
QYSTEVVWK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=845197 QSG86_RS02045 WP_317029985.1 216621..217370(-) (pilF) [Acinetobacter sp. SAAs474]
ATGCTTGGATGTCAAAATATACCAAATAATGCCTTAAAAGCAGATCCAGCAAAATCGCTACAAGCACATACTCAATTGGC
AGCAGAATATATCAAATCCGCCGACTATGATGCCGCATCAAGAGAATTGGATACTGCATTAGGGCTAAATGCACGTGATG
CCTATGCCAATATGATGATGGGAGTATTACTGCAGAACGAGGGGAGTCCTGCTAATATTGCCAAAGCTGAGCGATATTTT
AAAATGGCGATTGCTGTTGAGCCAAAAAATGCGCAAATCCGTAACAACTATGGTACGTATTTATATCAAACAGGCCGTTA
TAATGAAGCATTGGCACAATTGGAAATCGCAGGTTCAACATTAGGCTATGATCAGCGTTATACTGCACTTGAAAGTAAAG
GCAGAACTTACATGAAACTAGGCGACTTAACACATGCTGAATCGGCATTTCAACAGGCGCTTAATGTAAATCCAGCTTCA
TACATCGCAATGATTGAGCTCGCAGAAATTAAATATTTAGGCAACTCTATTCCTGCTGCAACCCAATTATATCACCAAGC
GGTAACACTGATTGGTGAAAAGAATTTAGATTCACATGCATTATGGATTGGTATCCGTATTGCACGTGCCAATGGCAATT
CATTAGAAAGACAAGTGCTTGTAAATCAGTTGCGAGCTTTGTACCCTTCTAGTCTAGAATATCAACGTTATTTGCAATTA
CAGTACAGTACTGAGGTCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(72-130)

Tetratricopeptide repeat (TPR_16)

(133-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

63.563

99.197

0.631
  pilF Acinetobacter baylyi ADP1

59.109

99.197

0.586